Abstract
This conference proceeding describes the culturing and expansion of mouse induced pluripotent stem cells (iPSCs) in hydrogel core-shell microfiber; the core consists of iPSCs and extracellular matrix (ECM) proteins, and the shell is composed of calcium alginate. This culture system is established by using a microfluidic co-axial device. The mouse iPSCs in this feeder-free 3D culture system expand with sustained pluripotency for nine days, even though their expansion ratio decrease to almost half of that when cultured by conventional 2D system on feeder cells. We believe that this feeder-free 3D culture system would be useful to obtain high-quality iPSCs to apply them in various fields including pharmaceutical and medicine, where contamination of feeder cells need to be avoided.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 910-911 |
| Number of pages | 2 |
| ISBN (Print) | 9780979806476 |
| Publication status | Published - 2014 |
| Externally published | Yes |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 2014 Oct 26 → 2014 Oct 30 |
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 14/10/26 → 14/10/30 |
Keywords
- 3D culture system
- Core-shell microfiber
- Induced pluripotent stem cell
- Pluripotency
ASJC Scopus subject areas
- Control and Systems Engineering