TY - GEN
T1 - Femto-liter protein purification by parallel two-phase nanofluidics
AU - Matsuura, Shu
AU - Kazoe, Yutaka
AU - Kitamori, Takehiko
N1 - Funding Information:
This work was supported by JST CREST Grant Number JPMJCR14G1, Japan
Funding Information:
This research was supported by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health, Award Number R03EB028043. The content is solely the responsibility of the authors.
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - We succeeded in the formation of aqueous parallel two-phase flow in nanochannel for the first time and applied it to fL protein purification. As a result, the removal of 79% lipid and the remains of 91% protein were realized at two-phase contact time of 10 ms and the volume of 16 fL which was much smaller than the volume of single cell (pL). Therefore, this method will greatly contribute to development of pretreatment process in single cell protein analysis.
AB - We succeeded in the formation of aqueous parallel two-phase flow in nanochannel for the first time and applied it to fL protein purification. As a result, the removal of 79% lipid and the remains of 91% protein were realized at two-phase contact time of 10 ms and the volume of 16 fL which was much smaller than the volume of single cell (pL). Therefore, this method will greatly contribute to development of pretreatment process in single cell protein analysis.
KW - Aqueous two-phase system
KW - Nanochannel
KW - Nanofluidics
KW - Purification
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M3 - Conference contribution
AN - SCOPUS:85094950791
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 618
EP - 619
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -