Fine-tuning in mineral cross-linking of biopolymer nanoparticle for incorporation and release of cargo

Yuuka Fukui, Narumi Kabayama, Keiji Fujimoto

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We developed a mineral cross-linking strategy to prepare a biopolymer-based nanoparticle using calcium phosphate (CaP) as a cross-linker. Nanoparticles were first formed by mixing deoxyribonucleic acid (DNA) with cationic surfactants, and were cross-linked by CaP precipitation. After removal of the surfactants, we carried out the alternative dialysis of nanoparticles against CaCl<inf>2</inf> aqueous solution and phosphate buffered solution for further mineral cross-linking. XRD and FT-IR studies revealed that the resultant nanoparticles were produced by mineral cross-linkages of hydroxyapatite (HAp) and the crystal amount and properties such as morphology and crystallinity could be well-controlled by the reaction conditions. Chemical dyes could be incorporated into nanoparticles via their affinities with crystal faces of HAp and DNA. Their release was tunable by crystal amount and properties of mineral cross-linkages. Also, the release could be triggered by mineral dissolution in response to pH. Such a mineral cross-linking will open up a potential way to provide a nanoparticle with versatile functions such as cleavable cross-linking, binding affinity for cargos, and pH-responsive release.

Original languageEnglish
Article number7351
Pages (from-to)168-174
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Publication statusPublished - 2015 Dec 1


  • Alternative dialysis
  • Calcium phosphate
  • Cross-linking
  • DNA
  • Mineralization
  • Nanoparticle
  • Release control

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces


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