Flow control-based 3D μPADs for organophosphate pesticide detection

Quoc Trung Hua, Hiroyuki Shibata, Yuki Hiruta, Daniel Citterio

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Flow control-based paper devices have recently shown great potential for point-of-need analysis, since they allow for the easy operation of multi-step assays by minimizing user operation. In this work, a wax printing method was evaluated as a means to control liquid flow in 3D microfluidic paper-based analytical devices (μPADs). The resulting flow controlbased 3D μPADs were applied to determine paraoxon-ethyl as a typical organophosphate pesticide model system. The analytical procedure is as simple as applying a 200-μL sample solution, resulting in reproducible (relative standard deviation of colorimetric signals from 6 independently fabricated devices, 2.63%) colorimetric signals within 1 h of the assay time with the limit of detection (LOD) reaching 25.0 μg/L. Finally, results obtained for pesticide-spiked water samples analyzed by flow control-based 3D μPADs showed good agreement with those from a conventional HPLC analysis with UV detection.

Original languageEnglish
Pages (from-to)393-399
Number of pages7
Journalanalytical sciences
Issue number4
Publication statusPublished - 2019


  • 3D μPADs
  • Biosensor
  • Flow control
  • Organophosphates

ASJC Scopus subject areas

  • Analytical Chemistry


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