Follicle-stimulating hormone enhances recovery from low-dose doxorubicin-induced spermatogenic disorders in mice

Jun Hagiuda, Hiromichi Ishikawa, Satoru Kaneko, Masako Okazaki, Mototsugu Oya, Ken Nakagawa

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Purpose We aimed to investigate the effects of FSH for promoting spermatogenesis in mice with low-dose doxorubicininduced spermatogenesis impairment. Methods Eight-wk-old male imprinting control region mice were divided into three groups. Groups D and F received 0.5 mg/kg of doxorubicin twice weekly for 5 weeks. Group C received saline instead of doxorubicin. After inducing spermatogenesis impairment, group D was treated daily with saline for 4 weeks. Group F was given 1 IU of recombinant human FSH daily for 4 weeks. Spermatogenesis recovery was evaluated based on the testis weight, sperm count, histological assessment, and mating. The percentage of sperm with unfragmented deoxyribonucleic acid (DNA) was analyzed by single-cell pulsed-field gel electrophoresis, and the serum FSH levels were measured. Results The elevation of serum FSH advanced slowly. The testis weight, sperm count, percentage of seminiferous tubules with spermatogenesis, percentage of sperm with unfragmented DNA and pregnancy rate were significantly increased by the administration of FSH. Conclusion Our study findings indicated that the immediate administration of exogenous FSH can promote the recovery from impaired spermatogenesis induced by low-dose doxorubicin before endogenous FSH increases to the maximum level.

Original languageEnglish
Article numberA004
Pages (from-to)917-923
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
Issue number6
Publication statusPublished - 2015 Jun 1


  • Chemotherapy
  • Doxorubicin
  • FSH
  • Spermatogenesis impairment

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)


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