Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator

Ryota Kawamae; Atsushi Takata; Kenjiro Takemura; Yuta Kurashina

doi:10.1109/MEMS58180.2024.10439352

Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator

Ryota Kawamae, Atsushi Takata, Kenjiro Takemura, Yuta Kurashina

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The spheroid formation is essential in current regenerative medicine and drug screening because it can reproduce the function of tissues in vivo, and various methods have been reported for its formation. However, a method to form spheroids in large quantities and at high speed has not been established in conventional methods. In this study, we propose a method to form spheroids massively and rapidly by combining a pneumatic soft actuator molded with polydimethylsiloxane (PDMS) and agarose gel, then applying pressure to a cell mass. In this report, osteoblasts were cultured in the device with and without applying air pressure for 12 hours to form spheroids.

Original language	English
Title of host publication	IEEE 37th International Conference on Micro Electro Mechanical Systems, MEMS 2024
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	334-335
Number of pages	2
ISBN (Electronic)	9798350357929
DOIs	https://doi.org/10.1109/MEMS58180.2024.10439352
Publication status	Published - 2024
Event	37th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2024 - Austin, United States Duration: 2024 Jan 21 → 2024 Jan 25

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	37th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2024
Country/Territory	United States
City	Austin
Period	24/1/21 → 24/1/25

Keywords

Agarose gel
Pneumatic drive
Polydimethylsiloxane (PDMS)
Soft actuator
Spheroid

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMS58180.2024.10439352

Cite this

Kawamae, R., Takata, A., Takemura, K., & Kurashina, Y. (2024). Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator. In IEEE 37th International Conference on Micro Electro Mechanical Systems, MEMS 2024 (pp. 334-335). (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS58180.2024.10439352

Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator. / Kawamae, Ryota; Takata, Atsushi; Takemura, Kenjiro et al.
IEEE 37th International Conference on Micro Electro Mechanical Systems, MEMS 2024. Institute of Electrical and Electronics Engineers Inc., 2024. p. 334-335 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kawamae, R, Takata, A, Takemura, K & Kurashina, Y 2024, Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator. in IEEE 37th International Conference on Micro Electro Mechanical Systems, MEMS 2024. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 334-335, 37th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2024, Austin, United States, 24/1/21. https://doi.org/10.1109/MEMS58180.2024.10439352

Kawamae R, Takata A, Takemura K, Kurashina Y. Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator. In IEEE 37th International Conference on Micro Electro Mechanical Systems, MEMS 2024. Institute of Electrical and Electronics Engineers Inc. 2024. p. 334-335. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS58180.2024.10439352

Kawamae, Ryota ; Takata, Atsushi ; Takemura, Kenjiro et al. / Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator. IEEE 37th International Conference on Micro Electro Mechanical Systems, MEMS 2024. Institute of Electrical and Electronics Engineers Inc., 2024. pp. 334-335 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "The spheroid formation is essential in current regenerative medicine and drug screening because it can reproduce the function of tissues in vivo, and various methods have been reported for its formation. However, a method to form spheroids in large quantities and at high speed has not been established in conventional methods. In this study, we propose a method to form spheroids massively and rapidly by combining a pneumatic soft actuator molded with polydimethylsiloxane (PDMS) and agarose gel, then applying pressure to a cell mass. In this report, osteoblasts were cultured in the device with and without applying air pressure for 12 hours to form spheroids.",

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Forming Spheroid Using Hydrogel Microwell and Pneumatic Soft Actuator

Abstract

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Keywords

ASJC Scopus subject areas

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