Four genes for the calpain family locate on four distinct human chromosomes

Shigeo Ohno; S. Minoshima; J. Kudoh; R. Fukuyama; Y. Shimizu; S. Ohmi-Imajoh; N. Shimizu; K. Suzuki

doi:10.1159/000132937

Four genes for the calpain family locate on four distinct human chromosomes

Shigeo Ohno, S. Minoshima, J. Kudoh, R. Fukuyama, Y. Shimizu, S. Ohmi-Imajoh, N. Shimizu, K. Suzuki

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Calcium dependent proteases (calpains. CAPNs, E.C.3.4.22.17) constitute a family of proteins which share a homologous cysteine-protease domain (large subunits. LI. L2. and L3) and an E-F hand Ca²⁺-binding domain (L1. L2. L3. and small subunit. S). We have mapped the genes for four calpain proteins (LI, L2, L3, and S) on four distinct human chromosomes by a combination of spot-blot hybridization to flow-sorted chromosomes and Southern hybridization of DNAs from a human × mouse hybrid cell panel. The genes for calpain LI (CAPN1. large subunit of calpain I). L2 (CAPN2, large subunit of calpain II), L3 (CAPN3. a protein related to the large subunits), and S (CAPN4. a small subunit common to calpains I and II) were assigned to human chromosomes 11, 1.15. and 19. respectively.

Original language	English
Pages (from-to)	225-229
Number of pages	5
Journal	Cytogenetics and Cell Genetics
Volume	53
Issue number	4
DOIs	https://doi.org/10.1159/000132937
Publication status	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Genetics
Cell Biology

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title = "Four genes for the calpain family locate on four distinct human chromosomes",

abstract = "Calcium dependent proteases (calpains. CAPNs, E.C.3.4.22.17) constitute a family of proteins which share a homologous cysteine-protease domain (large subunits. LI. L2. and L3) and an E-F hand Ca2+-binding domain (L1. L2. L3. and small subunit. S). We have mapped the genes for four calpain proteins (LI, L2, L3, and S) on four distinct human chromosomes by a combination of spot-blot hybridization to flow-sorted chromosomes and Southern hybridization of DNAs from a human × mouse hybrid cell panel. The genes for calpain LI (CAPN1. large subunit of calpain I). L2 (CAPN2, large subunit of calpain II), L3 (CAPN3. a protein related to the large subunits), and S (CAPN4. a small subunit common to calpains I and II) were assigned to human chromosomes 11, 1.15. and 19. respectively.",

author = "Shigeo Ohno and S. Minoshima and J. Kudoh and R. Fukuyama and Y. Shimizu and S. Ohmi-Imajoh and N. Shimizu and K. Suzuki",

year = "1990",

doi = "10.1159/000132937",

language = "English",

volume = "53",

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journal = "Cytogenetics and Cell Genetics",

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TY - JOUR

T1 - Four genes for the calpain family locate on four distinct human chromosomes

AU - Ohno, Shigeo

AU - Minoshima, S.

AU - Kudoh, J.

AU - Fukuyama, R.

AU - Shimizu, Y.

AU - Ohmi-Imajoh, S.

AU - Shimizu, N.

AU - Suzuki, K.

PY - 1990

Y1 - 1990

N2 - Calcium dependent proteases (calpains. CAPNs, E.C.3.4.22.17) constitute a family of proteins which share a homologous cysteine-protease domain (large subunits. LI. L2. and L3) and an E-F hand Ca2+-binding domain (L1. L2. L3. and small subunit. S). We have mapped the genes for four calpain proteins (LI, L2, L3, and S) on four distinct human chromosomes by a combination of spot-blot hybridization to flow-sorted chromosomes and Southern hybridization of DNAs from a human × mouse hybrid cell panel. The genes for calpain LI (CAPN1. large subunit of calpain I). L2 (CAPN2, large subunit of calpain II), L3 (CAPN3. a protein related to the large subunits), and S (CAPN4. a small subunit common to calpains I and II) were assigned to human chromosomes 11, 1.15. and 19. respectively.

AB - Calcium dependent proteases (calpains. CAPNs, E.C.3.4.22.17) constitute a family of proteins which share a homologous cysteine-protease domain (large subunits. LI. L2. and L3) and an E-F hand Ca2+-binding domain (L1. L2. L3. and small subunit. S). We have mapped the genes for four calpain proteins (LI, L2, L3, and S) on four distinct human chromosomes by a combination of spot-blot hybridization to flow-sorted chromosomes and Southern hybridization of DNAs from a human × mouse hybrid cell panel. The genes for calpain LI (CAPN1. large subunit of calpain I). L2 (CAPN2, large subunit of calpain II), L3 (CAPN3. a protein related to the large subunits), and S (CAPN4. a small subunit common to calpains I and II) were assigned to human chromosomes 11, 1.15. and 19. respectively.

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DO - 10.1159/000132937

M3 - Article

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AN - SCOPUS:0025183807

SN - 0301-0171

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EP - 229

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

IS - 4

ER -

Four genes for the calpain family locate on four distinct human chromosomes

Abstract

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