TY - JOUR
T1 - Fragmin/protamine microparticles (F/P MPs) as cell carriers enhance the formation and growth of tumors In Vivo
AU - Kumano, Isao
AU - Kishimoto, Satoko
AU - Nakamura, Shingo
AU - Hattori, Hidemi
AU - Tanaka, Yoshihiro
AU - Nakata, Mitsuhiro
AU - Sato, Toshinori
AU - Fujita, Masanori
AU - Maehara, Tadaaki
AU - Ishihara, Masayuki
N1 - Funding Information:
1Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan; 2Department of Biosciences and Informatics, School of Fundamental Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, Kanagawa 223-8522, Japan; 3Japan Society for the Promotion of Science, Tokyo, Japan; and 4Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan
PY - 2011/9
Y1 - 2011/9
N2 - Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of waterinsoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5-3 lm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 9 105 Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation.
AB - Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of waterinsoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5-3 lm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 9 105 Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation.
KW - Cell carrier
KW - Cell/F/P MP aggregate
KW - Fragmin/protamine microparticles
KW - Tumor cell transplantation
KW - Tumor growth
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U2 - 10.1007/s12195-011-0172-0
DO - 10.1007/s12195-011-0172-0
M3 - Article
AN - SCOPUS:81855194115
SN - 1865-5025
VL - 4
SP - 476
EP - 483
JO - Cellular and Molecular Bioengineering
JF - Cellular and Molecular Bioengineering
IS - 3
ER -
