TY - JOUR
T1 - Functional cloning and characterization of a multidrug efflux pump, MexHI-OpmD, from a Pseudomonas aeruginosa mutant
AU - Sekiya, Hiroshi
AU - Mima, Takehiko
AU - Morita, Yuji
AU - Kuroda, Teruo
AU - Mizushima, Tohru
AU - Tsuchiya, Tomofusa
PY - 2003/9/1
Y1 - 2003/9/1
N2 - We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.
AB - We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.
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U2 - 10.1128/AAC.47.9.2990-2992.2003
DO - 10.1128/AAC.47.9.2990-2992.2003
M3 - Article
C2 - 12937010
AN - SCOPUS:0041421188
SN - 0066-4804
VL - 47
SP - 2990
EP - 2992
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 9
ER -
