Functional comparison of transactivation by human retrovirus rev and rex genes

Hiroyuki Sakai, Haruhiko Siomi, Hisatoshi Shida, Riri Shibata, Takahiro Kiyomasu, Akio Adach

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)


The effect of rev-responsive element deletion on human immunodeficiency virus type 1 (HIV-1) type 2 (HIV-2) gene expression was examined. The phenotypes of HIV-1 and HIV-2 provirus DNAs lacking the rev-responsive element, as determined by transfection experiments, were indistinguishable from those of virus DNAs carrying rev gene mutations. By using rev-responsive elements derived from these two viruses, we developed two monitoring systems to evaluate the functionality of HIV-1 rev, HIV-2 rev, and human T-Iymphotropic virus type I rex. In both systems, HIV-1 rev and human T-lymphotropic virus type I rex transactivated HIV-2 very efficiently. On the contrary, HIV-2 rev T-lymphotropic virus type I rex were poor activators of HIV-1. No functional replacement of rex by HIV-2 rev was observed.

Original languageEnglish
Pages (from-to)5833-5839
Number of pages7
JournalJournal of Virology
Issue number12
Publication statusPublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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