Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells

Hashimoto Yukiya Hashimoto, Ozaki Junko Ozaki, Yasuhara Masato Yasuhara, Hori Ryohei Hori, Suga Shin-ichi Suga, Itoh Hiroshi Itoh, Nakao Kazuwa Nakao, Inui Ken-inchi Inui

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of α-human atrial natriuretic peptide (α-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with α-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for α-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.

Original languageEnglish
Pages (from-to)443-445
Number of pages3
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Issue number3
Publication statusPublished - 1994 Aug 16
Externally publishedYes


  • ANP (atrial natriuretic peptide)
  • Guanylate cyclase
  • LLC-PK cell
  • Natriuretic peptide, C-type

ASJC Scopus subject areas

  • Pharmacology


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