Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system

Kwang Wook Ko; Fengqiu Lin; Takumi Katsumata; Yoshinori Sugai; Sho Miyazaki; Hiroshi Kawaide; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane

doi:10.1271/bbb.80427

Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system

Kwang Wook Ko, Fengqiu Lin, Takumi Katsumata, Yoshinori Sugai, Sho Miyazaki, Hiroshi Kawaide, Kazunori Okada, Hideaki Nojiri, Hisakazu Yamane

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

Original language	English
Pages (from-to)	3285-3288
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	12
DOIs	https://doi.org/10.1271/bbb.80427
Publication status	Published - 2008
Externally published	Yes

Keywords

Cytochrome P450 monooxygenase (P450)
Ent-kaurene oxidase
Rice

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.80427

Cite this

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abstract = "Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.",

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year = "2008",

doi = "10.1271/bbb.80427",

language = "English",

volume = "72",

pages = "3285--3288",

journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system

AU - Ko, Kwang Wook

AU - Lin, Fengqiu

AU - Katsumata, Takumi

AU - Sugai, Yoshinori

AU - Miyazaki, Sho

AU - Kawaide, Hiroshi

AU - Okada, Kazunori

AU - Nojiri, Hideaki

AU - Yamane, Hisakazu

PY - 2008

Y1 - 2008

N2 - Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

AB - Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

KW - Cytochrome P450 monooxygenase (P450)

KW - Ent-kaurene oxidase

KW - Rice

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SN - 0916-8451

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

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ER -

Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system

Abstract

Keywords

ASJC Scopus subject areas

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