TY - JOUR
T1 - GAD-reactive T cells were mainly detected in autoimmune-related type 1 diabetic patients with HLA DR9
AU - Itoh, Arata
AU - Shimada, Akira
AU - Kodama, Keiichi
AU - Morimoto, Jiro
AU - Suzuki, Ryuji
AU - Oikawa, Yoichi
AU - Irie, Junichiro
AU - Nakagawa, Yoshinori
AU - Shigihara, Toshikatsu
AU - Kanazawa, Yasuhiko
AU - Okubo, Yoshiaki
AU - Motohashi, Yoshiko
AU - Maruyama, Taro
AU - Saruta, Takao
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Type 1 diabetes mellitus (T1DM) is considered to be a T cell-mediated disease, and many reports suggest that some HLA types, especially HLA DR4 and DR9, convey susceptibility to T1DM in Japanese. We investigated the association between T cell reactivity against GAD and HLA types in "islet-associated autoantibody-positive" T1DM in Japanese. Blood samples were obtained from 36 "autoantibody-positive" type 1 diabetic patients with HLA DR4 or DR9 and 23 type 2 diabetic patients with HLA DR4 or DR9 as controls. They were divided into three groups, DR4/9, DR4/X, and DR9/X groups. In each HLA type group, GAD-reactive IFN-γ-preducing CD4+ cells were assessed by means of intracellular cytokine staining for flow cytometry. Type 1 diabetic patients with HLA DR9/X had significantly higher numbers of GAD-reactive IFN-γ-producing CD4+ cells as compared to type 1 diabetic patients with DR4/X or DR4/9 (P < 0.05) and all type 2 diabetic patients. There was no significant difference in the number of GAD-reactive IFN-γ-producing CD4+ cells between type 1 diabetic and type 2 diabetic patients belonging to the DR4/X and DR4/9 groups. There was an association between T cell reactivity against GAD and HLA DR9 in Japanese type 1 diabetes.
AB - Type 1 diabetes mellitus (T1DM) is considered to be a T cell-mediated disease, and many reports suggest that some HLA types, especially HLA DR4 and DR9, convey susceptibility to T1DM in Japanese. We investigated the association between T cell reactivity against GAD and HLA types in "islet-associated autoantibody-positive" T1DM in Japanese. Blood samples were obtained from 36 "autoantibody-positive" type 1 diabetic patients with HLA DR4 or DR9 and 23 type 2 diabetic patients with HLA DR4 or DR9 as controls. They were divided into three groups, DR4/9, DR4/X, and DR9/X groups. In each HLA type group, GAD-reactive IFN-γ-preducing CD4+ cells were assessed by means of intracellular cytokine staining for flow cytometry. Type 1 diabetic patients with HLA DR9/X had significantly higher numbers of GAD-reactive IFN-γ-producing CD4+ cells as compared to type 1 diabetic patients with DR4/X or DR4/9 (P < 0.05) and all type 2 diabetic patients. There was no significant difference in the number of GAD-reactive IFN-γ-producing CD4+ cells between type 1 diabetic and type 2 diabetic patients belonging to the DR4/X and DR4/9 groups. There was an association between T cell reactivity against GAD and HLA DR9 in Japanese type 1 diabetes.
KW - Cytokine
KW - GAD
KW - HLA
KW - T cell
KW - Type 1 diabetes
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U2 - 10.1196/annals.1337.006
DO - 10.1196/annals.1337.006
M3 - Article
C2 - 15699491
AN - SCOPUS:20044377032
SN - 0077-8923
VL - 1037
SP - 33
EP - 40
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -
