TY - JOUR
T1 - Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus
AU - Imaizumi, Yoichi
AU - Sakaguchi, Masanori
AU - Morishita, Tsuyoshi
AU - Ito, Mamoru
AU - Poirier, Françoise
AU - Sawamoto, Kazunobu
AU - Okano, Hideyuki
N1 - Funding Information:
We thank K. Nakashima, F.H. Gage, K. Sakurada, T. Shingo, K. Hayama, J. Hirabayashi, N. Nishi, T. Kadoya, H. Horie, M. Maekawa, N. Osumi, T. Kojima, O. Yamada, J. Yamane, and H Tada for technical assistance; and M. Ito, K. Fujita, M. Shiota, and A. Hirayama for secretarial assistance. This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Core Research for Evolutional Science and Technology (CREST), and Solution-Oriented Research for Science and Technology (SORST) of the Japan Science and Technology Agency (JST) to H.O., a Start-up research grant from the Japan Society for the Promotion of Science (JSPS), JSPS Postdoctoral Fellowships for Research Abroad, Naito Memorial Foundation research grant to M.S., Grant-in-Aid for Encouragement of Young Medical Scientists from Keio University, and Initiatives for Attractive Education in Graduate Schools from JSPS to Y.I.
PY - 2011
Y1 - 2011
N2 - Background. In the adult mammalian brain, neural stem cells (NSCs) proliferate in the dentate gyrus (DG) of the hippocampus and generate new neurons throughout life. A multimodal protein, Galectin-1, is expressed in neural progenitor cells (NPCs) and implicated in the proliferation of the NPCs in the DG. However, little is known about its detailed expression profile in the NPCs and functions in adult neurogenesis in the DG. Results. Our immunohistochemical and morphological analysis showed that Galectin-1 was expressed in the type 1 and 2a cells, which are putative NSCs, in the subgranular zone (SGZ) of the adult mouse DG. To study Galectin-1's function in adult hippocampal neurogenesis, we made galectin-1 knock-out mice on the C57BL6 background and characterized the effects on neurogenesis. In the SGZ of the galectin-1 knock-out mice, increased numbers of type 1 cells, DCX-positive immature progenitors, and NeuN-positive newborn neurons were observed. Using triple-labeling immunohistochemistry and morphological analyses, we found that the proliferation of the type-1 cells was increased in the SGZ of the galectin-1 knock-out mice, and we propose that this proliferation is the mechanism for the net increase in the adult neurogenesis in these knock-out mice DG. Conclusions. Galectin-1 is expressed in the neural stem cells and down-regulates neurogenesis in the adult hippocampus.
AB - Background. In the adult mammalian brain, neural stem cells (NSCs) proliferate in the dentate gyrus (DG) of the hippocampus and generate new neurons throughout life. A multimodal protein, Galectin-1, is expressed in neural progenitor cells (NPCs) and implicated in the proliferation of the NPCs in the DG. However, little is known about its detailed expression profile in the NPCs and functions in adult neurogenesis in the DG. Results. Our immunohistochemical and morphological analysis showed that Galectin-1 was expressed in the type 1 and 2a cells, which are putative NSCs, in the subgranular zone (SGZ) of the adult mouse DG. To study Galectin-1's function in adult hippocampal neurogenesis, we made galectin-1 knock-out mice on the C57BL6 background and characterized the effects on neurogenesis. In the SGZ of the galectin-1 knock-out mice, increased numbers of type 1 cells, DCX-positive immature progenitors, and NeuN-positive newborn neurons were observed. Using triple-labeling immunohistochemistry and morphological analyses, we found that the proliferation of the type-1 cells was increased in the SGZ of the galectin-1 knock-out mice, and we propose that this proliferation is the mechanism for the net increase in the adult neurogenesis in these knock-out mice DG. Conclusions. Galectin-1 is expressed in the neural stem cells and down-regulates neurogenesis in the adult hippocampus.
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U2 - 10.1186/1756-6606-4-7
DO - 10.1186/1756-6606-4-7
M3 - Article
C2 - 21269521
AN - SCOPUS:79251573229
SN - 1756-6606
VL - 4
JO - Molecular brain
JF - Molecular brain
IS - 1
M1 - 7
ER -