Gene expression profile of peripheral blood mononuclear cells may contribute to the identification and immunological classification of breast cancer patients

Eiji Suzuki, Masahiro Sugimoto, Kosuke Kawaguchi, Fengling Pu, Ryuji Uozumi, Ayane Yamaguchi, Mariko Nishie, Moe Tsuda, Takeshi Kotake, Satoshi Morita, Masakazu Toi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Background: It has been reported that the gene expression profile of peripheral blood mononuclear cells (PBMCs) exhibits a unique gene expression signature in several types of cancer. In this study, we aimed to explore the breast cancer patient-specific gene expression profile of PBMCs and discuss immunological insight on host antitumor immune responses. Methods: We comprehensively analyzed the gene expression of PBMCs by RNA sequencing in the breast cancer patients as compared to that of healthy volunteers (HVs). Pathway enrichment analysis was performed on MetaCore tm to search the molecular pathways associated with the gene expression profile of PBMCs in cancer patients compared with HVs. Results: We found a significant unique gene expression signature, such as the Toll-like receptor (TLR) 3- and TLR4-induced Toll/interleukin-1 receptor domain-containing adapter molecule 1 (TICAM1)-specific signaling pathway in the breast cancer patients as compared to that of healthy volunteers. Distinctive immunological gene expression profiles also showed the possibility of classifying breast cancer patients into subgroups such as T-cell inhibitory and monocyte-activating groups independent of known phenotypes of breast cancer. Conclusions: These preliminary findings suggest that evaluation of gene expression patterns of PBMCs might be both a less invasive diagnostic procedure and a useful way to reveal immunological insight of breast cancer, including biomarkers for cancer immunotherapy, such as immune checkpoint inhibitor therapy.

Original languageEnglish
Pages (from-to)282-289
Number of pages8
JournalBreast Cancer
Volume26
Issue number3
DOIs
Publication statusPublished - 2019 May 1
Externally publishedYes

Keywords

  • Gene expression profile
  • Immune checkpoint molecule
  • Immuno-oncology
  • Peripheral blood mononuclear cells
  • RNA sequencing

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Pharmacology (medical)

Fingerprint

Dive into the research topics of 'Gene expression profile of peripheral blood mononuclear cells may contribute to the identification and immunological classification of breast cancer patients'. Together they form a unique fingerprint.

Cite this