Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

Mayu Hashimoto; Minoru Morimoto; Hiroyuki Saimoto; Yoshihiro Shigemasa; Hironobu Yanagie; Masazumi Eriguchi; Toshinori Sato

doi:10.1007/s10529-006-9006-x

Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie, Masazumi Eriguchi, Toshinori Sato

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

Original language	English
Pages (from-to)	815-821
Number of pages	7
Journal	Biotechnology Letters
Volume	28
Issue number	11
DOIs	https://doi.org/10.1007/s10529-006-9006-x
Publication status	Published - 2006 Jun

Keywords

Chitosan
Gene transfer
Macrophage
Mannose
Plasmid DNA
Receptor-mediated endocytosis

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-006-9006-x

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title = "Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages",

abstract = "Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.",

keywords = "Chitosan, Gene transfer, Macrophage, Mannose, Plasmid DNA, Receptor-mediated endocytosis",

author = "Mayu Hashimoto and Minoru Morimoto and Hiroyuki Saimoto and Yoshihiro Shigemasa and Hironobu Yanagie and Masazumi Eriguchi and Toshinori Sato",

note = "Funding Information: Acknowledgements This work was partly supported by the Special Coordination of Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government (T.S.).",

year = "2006",

month = jun,

doi = "10.1007/s10529-006-9006-x",

language = "English",

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pages = "815--821",

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T1 - Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

AU - Hashimoto, Mayu

AU - Morimoto, Minoru

AU - Saimoto, Hiroyuki

AU - Shigemasa, Yoshihiro

AU - Yanagie, Hironobu

AU - Eriguchi, Masazumi

AU - Sato, Toshinori

N1 - Funding Information: Acknowledgements This work was partly supported by the Special Coordination of Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government (T.S.).

PY - 2006/6

Y1 - 2006/6

N2 - Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

AB - Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

KW - Chitosan

KW - Gene transfer

KW - Macrophage

KW - Mannose

KW - Plasmid DNA

KW - Receptor-mediated endocytosis

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DO - 10.1007/s10529-006-9006-x

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SN - 0141-5492

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

Abstract

Keywords

ASJC Scopus subject areas

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