TY - JOUR
T1 - Generation of induced pluripotent stem cells from a small amount of human peripheral blood using a combination of activated T cells and Sendai virus
AU - Seki, Tomohisa
AU - Yuasa, Shinsuke
AU - Fukuda, Keiichi
N1 - Funding Information:
acKnowleDGMents T.S. is a research fellow of the Japan Society for the Promotion of Science. This work was supported in part by research grants from the Ministry of Education, Science and Culture, Japan, and by a grant from the New Energy and Industrial Technology Development Organization.
PY - 2012/4
Y1 - 2012/4
N2 - Induced pluripotent stem cells (iPSCs) have become important cell sources for genetic disease models, and they have the potential to be cell sources for future clinical therapies. However, invasive tissue sampling reduces the number of candidates who consent to donate cells for iPSC generation. In addition, integrated transgenes can potentially insert at inappropriate points in the genome, and in turn have a direct oncogenic effect. Technical modifications using a combination of activated T cells and a temperature-sensitive mutant of Sendai virus (SeV) can avoid invasive tissue sampling and residual transgene issues in generating iPSCs. Such advances may increase the number of consenting patients for cell donations. Here we present a detailed protocol for the generation of iPSCs from a small amount of human peripheral blood using a combination of activated T cells and mutant SeV encoding human OCT3/4, SOX2, KLF4 and c-MYC; T cell-derived iPSCs can be generated within 1 month of blood sampling.
AB - Induced pluripotent stem cells (iPSCs) have become important cell sources for genetic disease models, and they have the potential to be cell sources for future clinical therapies. However, invasive tissue sampling reduces the number of candidates who consent to donate cells for iPSC generation. In addition, integrated transgenes can potentially insert at inappropriate points in the genome, and in turn have a direct oncogenic effect. Technical modifications using a combination of activated T cells and a temperature-sensitive mutant of Sendai virus (SeV) can avoid invasive tissue sampling and residual transgene issues in generating iPSCs. Such advances may increase the number of consenting patients for cell donations. Here we present a detailed protocol for the generation of iPSCs from a small amount of human peripheral blood using a combination of activated T cells and mutant SeV encoding human OCT3/4, SOX2, KLF4 and c-MYC; T cell-derived iPSCs can be generated within 1 month of blood sampling.
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U2 - 10.1038/nprot.2012.015
DO - 10.1038/nprot.2012.015
M3 - Article
C2 - 22422317
AN - SCOPUS:84861785745
SN - 1754-2189
VL - 7
SP - 718
EP - 728
JO - Nature Protocols
JF - Nature Protocols
IS - 4
ER -
