TY - JOUR
T1 - Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device
AU - Yamashita, Hitoyoshi
AU - Morita, Masamune
AU - Sugiura, Haruka
AU - Fujiwara, Kei
AU - Onoe, Hiroaki
AU - Takinoue, Masahiro
N1 - Funding Information:
We thank Mr. Masayuki Hayakawa (Tokyo Tech.) for their technical support. We also thank Prof. Hiroyuki Ohta as well as Prof. Hisakazu Mihara, Prof. Makio Tokunaga, Prof. Hiroyuki Ohtani, and Prof. Yasunori Aizawa (Tokyo Tech.) for the helpful discussions. This work was supported by the PRESTO “Design and Control of Cellular Functions” research area of Japan Science and Technology Agency (JST) , a Grant-in-Aid for Scientific Research on Innovative Areas “Molecular Robotics” (Project No. 24104002 ) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan , Grant-in-Aids for Young Scientists (A) (Project No. 24680033 ) and Scientific Research (B) (Project No. 26280097 ) from the Japan Society for the Promotion of Science (JSPS) , and the Creative Design for Bioscience and Biotechnology course of the School of Bioscience and Biotechnology at Tokyo Tech .
Publisher Copyright:
© 2014 The Society for Biotechnology, Japan.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments.
AB - We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments.
KW - Biochemical reactions
KW - Cell encapsulation
KW - Centrifuge
KW - Microfluidic device
KW - Monodisperse microdroplets
KW - Plateau-Rayleigh instability
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U2 - 10.1016/j.jbiosc.2014.09.018
DO - 10.1016/j.jbiosc.2014.09.018
M3 - Article
C2 - 25454697
AN - SCOPUS:84924584956
SN - 1389-1723
VL - 119
SP - 492
EP - 495
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -