Generation of mouse ES cell lines engineered for the forced induction of transcription factors

Lina S. Correa-Cerro; Yulan Piao; Alexei A. Sharov; Akira Nishiyama; Jean S. Cadet; Hong Yu; Lioudmila V. Sharova; Li Xin; Hien G. Hoang; Marshall Thomas; Yong Qian; Dawood B. Dudekula; Emily Meyers; Bernard Y. Binder; Gregory Mowrer; Uwem Bassey; Dan L. Longo; David Schlessinger; Minoru S.H. Ko

doi:10.1038/srep00167

Generation of mouse ES cell lines engineered for the forced induction of transcription factors

Lina S. Correa-Cerro, Yulan Piao, Alexei A. Sharov, Akira Nishiyama, Jean S. Cadet, Hong Yu, Lioudmila V. Sharova, Li Xin, Hien G. Hoang, Marshall Thomas, Yong Qian, Dawood B. Dudekula, Emily Meyers, Bernard Y. Binder, Gregory Mowrer, Uwem Bassey, Dan L. Longo, David Schlessinger, Minoru S.H. Ko

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.

Original language	English
Article number	167
Journal	Scientific reports
Volume	1
DOIs	https://doi.org/10.1038/srep00167
Publication status	Published - 2011
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1038/srep00167

Cite this

Correa-Cerro, L. S., Piao, Y., Sharov, A. A., Nishiyama, A., Cadet, J. S., Yu, H., Sharova, L. V., Xin, L., Hoang, H. G., Thomas, M., Qian, Y., Dudekula, D. B., Meyers, E., Binder, B. Y., Mowrer, G., Bassey, U., Longo, D. L., Schlessinger, D., & Ko, M. S. H. (2011). Generation of mouse ES cell lines engineered for the forced induction of transcription factors. Scientific reports, 1, Article 167. https://doi.org/10.1038/srep00167

Correa-Cerro, LS, Piao, Y, Sharov, AA, Nishiyama, A, Cadet, JS, Yu, H, Sharova, LV, Xin, L, Hoang, HG, Thomas, M, Qian, Y, Dudekula, DB, Meyers, E, Binder, BY, Mowrer, G, Bassey, U, Longo, DL, Schlessinger, D & Ko, MSH 2011, 'Generation of mouse ES cell lines engineered for the forced induction of transcription factors', Scientific reports, vol. 1, 167. https://doi.org/10.1038/srep00167

@article{cc90fe367dae488687c4c02e2570e391,

title = "Generation of mouse ES cell lines engineered for the forced induction of transcription factors",

abstract = "Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.",

author = "Correa-Cerro, {Lina S.} and Yulan Piao and Sharov, {Alexei A.} and Akira Nishiyama and Cadet, {Jean S.} and Hong Yu and Sharova, {Lioudmila V.} and Li Xin and Hoang, {Hien G.} and Marshall Thomas and Yong Qian and Dudekula, {Dawood B.} and Emily Meyers and Binder, {Bernard Y.} and Gregory Mowrer and Uwem Bassey and Longo, {Dan L.} and David Schlessinger and Ko, {Minoru S.H.}",

note = "Funding Information: This research was supported entirely by the Intramural Research Program of the NIH, National Institute on Aging.",

year = "2011",

doi = "10.1038/srep00167",

language = "English",

volume = "1",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Generation of mouse ES cell lines engineered for the forced induction of transcription factors

AU - Correa-Cerro, Lina S.

AU - Piao, Yulan

AU - Sharov, Alexei A.

AU - Nishiyama, Akira

AU - Cadet, Jean S.

AU - Yu, Hong

AU - Sharova, Lioudmila V.

AU - Xin, Li

AU - Hoang, Hien G.

AU - Thomas, Marshall

AU - Qian, Yong

AU - Dudekula, Dawood B.

AU - Meyers, Emily

AU - Binder, Bernard Y.

AU - Mowrer, Gregory

AU - Bassey, Uwem

AU - Longo, Dan L.

AU - Schlessinger, David

AU - Ko, Minoru S.H.

N1 - Funding Information: This research was supported entirely by the Intramural Research Program of the NIH, National Institute on Aging.

PY - 2011

Y1 - 2011

N2 - Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.

AB - Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.

UR - http://www.scopus.com/inward/record.url?scp=84859736980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859736980&partnerID=8YFLogxK

U2 - 10.1038/srep00167

DO - 10.1038/srep00167

M3 - Article

C2 - 22355682

AN - SCOPUS:84859736980

SN - 2045-2322

VL - 1

JO - Scientific reports

JF - Scientific reports

M1 - 167

ER -

Generation of mouse ES cell lines engineered for the forced induction of transcription factors

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this