TY - JOUR
T1 - Genetic mutation analysis of the malignant transformation of sinonasal inverted papilloma by targeted amplicon sequencing
AU - Yasukawa, Shinichiro
AU - Kano, Satoshi
AU - Hatakeyama, Hiromitsu
AU - Nakamaru, Yuji
AU - Takagi, Dai
AU - Mizumachi, Takatsugu
AU - Suzuki, Masanobu
AU - Suzuki, Takayoshi
AU - Nakazono, Akira
AU - Tanaka, Shinya
AU - Nishihara, Hiroshi
AU - Homma, Akihiro
N1 - Publisher Copyright:
© 2018, Japan Society of Clinical Oncology.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Background: The mechanism underlying the malignant transformation of inverted papilloma (IP) has not yet been elucidated. Methods: To clarify the genes responsible for the malignant transformation, we analyzed 10 cases of IP, 8 of IP with dysplasia, and 11 of squamous cell carcinoma (SCC) by targeted amplicon sequencing. Results: The number of mutant genes increased in the order of IP < dysplasia < SCC. Significant differences were observed in the mutation rates of three genes (KRAS, APC and STK11) in particular. TP53 was altered frequently in each group and might be involved in malignant transformation based on to the site of the mutation. A comparison of the genetic variants by region of IP tissue among patients with IP alone, and those with dysplasia or SCC revealed significant differences in the mutation rate of the KRAS gene. Conclusion: Identification of genetic mutations in KRAS is effective for predicting the malignant transformation of IP.
AB - Background: The mechanism underlying the malignant transformation of inverted papilloma (IP) has not yet been elucidated. Methods: To clarify the genes responsible for the malignant transformation, we analyzed 10 cases of IP, 8 of IP with dysplasia, and 11 of squamous cell carcinoma (SCC) by targeted amplicon sequencing. Results: The number of mutant genes increased in the order of IP < dysplasia < SCC. Significant differences were observed in the mutation rates of three genes (KRAS, APC and STK11) in particular. TP53 was altered frequently in each group and might be involved in malignant transformation based on to the site of the mutation. A comparison of the genetic variants by region of IP tissue among patients with IP alone, and those with dysplasia or SCC revealed significant differences in the mutation rate of the KRAS gene. Conclusion: Identification of genetic mutations in KRAS is effective for predicting the malignant transformation of IP.
KW - Inverted papilloma
KW - Malignant transformation
KW - NGS
KW - Squamous cell carcinoma
KW - Targeted amplicon sequence
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U2 - 10.1007/s10147-018-1296-1
DO - 10.1007/s10147-018-1296-1
M3 - Article
C2 - 29779136
AN - SCOPUS:85047110054
SN - 1341-9625
VL - 23
SP - 835
EP - 843
JO - International Journal of Clinical Oncology
JF - International Journal of Clinical Oncology
IS - 5
ER -