Glucose transport in interlobular ducts isolated from rat pancreas

Sachiko Futakuchi, Hiroshi Ishiguro, Satoru Naruse, Shigeru B.H. Ko, Kotoyo Fujiki, Akiko Yamamoto, Miyuki Nakakuki, Ying Song, Martin C. Steward, Takaharu Kondo, Hidemi Goto

Research output: Contribution to journalArticlepeer-review


Pancreatic duct cells express Na+-dependent glucose transporter, SGLT1 and Na+-independent glucose transporters, GLUT1, GLUT2, and GLUT8. We examined transepithelial glucose transport by pancreatic duct. Interlobular ducts were isolated from rat pancreas. During overnight culture both ends of the duct segments sealed spontaneously. The lumen of the sealed duct was micropunctured and the luminal fluid was replaced by HEPES-buffered solution containing 10.0 mM or 44.4 mM glucose. The bath was perfused with HEPES-buffered solution at 37°Containing 10.0 or 44.4 mM glucose. Transepithelial differences in osmolality were balanced with mannitol. Glucose transport across ductal epithelium was measured by monitoring changes in luminal volume. When the lumen was filled with 44.4 mM glucose, with either 10.0 or 44.4 mM glucose in the bath, the luminal volume decreased to 65-0% of the initial volume in 15 min. Luminally-injected phlorizin, an inhibitor of SGLT1, abolished the decrease in luminal volume. With 10.0 mM glucose in the lumen and 44.4 mM glucose in the bath, the luminal volume did not change significantly. Luminal application of phlorizin under identical condition led to an increase in luminal volume. The data suggest that both active and passive transport mechanisms of glucose are present in pancreatic ductal epithelium.

Original languageEnglish
Pages (from-to)308-311
Number of pages4
JournalJournal of Medical Investigation
Issue numberSUPPL. 1
Publication statusPublished - 2009
Externally publishedYes


  • Glucose transport
  • Pancreatic duct cell
  • SGLT1

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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