Glycoreplica peptides

Teruhiko Matsubara; Toshinori Sato; Takao Taki

doi:10.1007/978-1-4939-8552-4_23

Glycoreplica peptides

Teruhiko Matsubara
, Toshinori Sato
, Takao Taki

Department of Biosciences and Informatics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

“Glycoreplica peptides” are prepared using a phage display peptide library and monoclonal antibodies that recognize the carbohydrate epitopes of glycoconjugate antigens. The peptides obtained not only mimic the shapes of original glycoconjugate antigens but also have some of their functions. We herein describe how to identify the amino acid alignments of glycoreplica peptides using phage display selection against carbohydrate-binding proteins. Target-specific peptides and proteins may be selected from the large repertory of a peptide/protein library using phage display technology. Glycoreplica peptides have the potential to become alternatives to carbohydrate ligands such as mimotopes for vaccinations and carbohydrate-derived drugs for carbohydrate-related diseases.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	437-447
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-8552-4_23
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1804
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Anti-carbohydrate antibody
Glycosidase inhibitor
Lectin
Phage display technology
Random peptide library

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-8552-4_23

Cite this

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title = "Glycoreplica peptides",

abstract = "“Glycoreplica peptides” are prepared using a phage display peptide library and monoclonal antibodies that recognize the carbohydrate epitopes of glycoconjugate antigens. The peptides obtained not only mimic the shapes of original glycoconjugate antigens but also have some of their functions. We herein describe how to identify the amino acid alignments of glycoreplica peptides using phage display selection against carbohydrate-binding proteins. Target-specific peptides and proteins may be selected from the large repertory of a peptide/protein library using phage display technology. Glycoreplica peptides have the potential to become alternatives to carbohydrate ligands such as mimotopes for vaccinations and carbohydrate-derived drugs for carbohydrate-related diseases.",

keywords = "Anti-carbohydrate antibody, Glycosidase inhibitor, Lectin, Phage display technology, Random peptide library",

author = "Teruhiko Matsubara and Toshinori Sato and Takao Taki",

note = "Funding Information: This study was supported by the Japan Society for the Promotion of Science Kakenhi grants (no. 14380411 and 17300159 to T.S.; no. 15 K01806 to T.M.). Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",

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doi = "10.1007/978-1-4939-8552-4\_23",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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TY - CHAP

T1 - Glycoreplica peptides

AU - Matsubara, Teruhiko

AU - Sato, Toshinori

AU - Taki, Takao

N1 - Funding Information: This study was supported by the Japan Society for the Promotion of Science Kakenhi grants (no. 14380411 and 17300159 to T.S.; no. 15 K01806 to T.M.). Publisher Copyright: © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2018

Y1 - 2018

N2 - “Glycoreplica peptides” are prepared using a phage display peptide library and monoclonal antibodies that recognize the carbohydrate epitopes of glycoconjugate antigens. The peptides obtained not only mimic the shapes of original glycoconjugate antigens but also have some of their functions. We herein describe how to identify the amino acid alignments of glycoreplica peptides using phage display selection against carbohydrate-binding proteins. Target-specific peptides and proteins may be selected from the large repertory of a peptide/protein library using phage display technology. Glycoreplica peptides have the potential to become alternatives to carbohydrate ligands such as mimotopes for vaccinations and carbohydrate-derived drugs for carbohydrate-related diseases.

AB - “Glycoreplica peptides” are prepared using a phage display peptide library and monoclonal antibodies that recognize the carbohydrate epitopes of glycoconjugate antigens. The peptides obtained not only mimic the shapes of original glycoconjugate antigens but also have some of their functions. We herein describe how to identify the amino acid alignments of glycoreplica peptides using phage display selection against carbohydrate-binding proteins. Target-specific peptides and proteins may be selected from the large repertory of a peptide/protein library using phage display technology. Glycoreplica peptides have the potential to become alternatives to carbohydrate ligands such as mimotopes for vaccinations and carbohydrate-derived drugs for carbohydrate-related diseases.

KW - Anti-carbohydrate antibody

KW - Glycosidase inhibitor

KW - Lectin

KW - Phage display technology

KW - Random peptide library

UR - https://www.scopus.com/pages/publications/85048946972

UR - https://www.scopus.com/pages/publications/85048946972#tab=citedBy

U2 - 10.1007/978-1-4939-8552-4_23

DO - 10.1007/978-1-4939-8552-4_23

M3 - Chapter

C2 - 29926424

AN - SCOPUS:85048946972

T3 - Methods in Molecular Biology

SP - 437

EP - 447

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Glycoreplica peptides

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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