Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury

Soya Kawabata; Morito Takano; Yuko Numasawa-Kuroiwa; Go Itakura; Yoshiomi Kobayashi; Yuichiro Nishiyama; Keiko Sugai; Soraya Nishimura; Hiroki Iwai; Miho Isoda; Shinsuke Shibata; Jun Kohyama; Akio Iwanami; Yoshiaki Toyama; Morio Matsumoto; Masaya Nakamura; Hideyuki Okano

doi:10.1016/j.stemcr.2015.11.013

Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury

Soya Kawabata, Morito Takano, Yuko Numasawa-Kuroiwa, Go Itakura, Yoshiomi Kobayashi, Yuichiro Nishiyama, Keiko Sugai, Soraya Nishimura, Hiroki Iwai, Miho Isoda, Shinsuke Shibata, Jun Kohyama, Akio Iwanami, Yoshiaki Toyama, Morio Matsumoto, Masaya Nakamura, Hideyuki Okano

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130 Citations (Scopus)

Abstract

Murine- and human-induced pluripotent stem cell-derived neural stem/progenitor cells (iPSC-NS/PCs) promote functional recovery following transplantation into the injured spinal cord in rodents and primates. Although remyelination of spared demyelinated axons is a critical mechanism in the regeneration of the injured spinal cord, human iPSC-NS/PCs predominantly differentiate into neurons both in vitro and in vivo. We therefore took advantage of our recently developed protocol to obtain human-induced pluripotent stem cell-derived oligodendrocyte precursor cell-enriched neural stem/progenitor cells and report the benefits of transplanting these cells in a spinal cord injury (SCI) model. We describe how this approach contributes to the robust remyelination of demyelinated axons and facilitates functional recovery after SCI.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Stem cell reports
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/j.stemcr.2015.11.013
Publication status	Published - 2016 Jan 12

ASJC Scopus subject areas

Biochemistry
Genetics
Developmental Biology
Cell Biology

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Kawabata, S., Takano, M., Numasawa-Kuroiwa, Y., Itakura, G., Kobayashi, Y., Nishiyama, Y., Sugai, K., Nishimura, S., Iwai, H., Isoda, M., Shibata, S., Kohyama, J., Iwanami, A., Toyama, Y., Matsumoto, M., Nakamura, M., & Okano, H. (2016). Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury. Stem cell reports, 6(1), 1-8. https://doi.org/10.1016/j.stemcr.2015.11.013

Kawabata, S, Takano, M, Numasawa-Kuroiwa, Y, Itakura, G, Kobayashi, Y, Nishiyama, Y, Sugai, K, Nishimura, S, Iwai, H, Isoda, M, Shibata, S, Kohyama, J, Iwanami, A, Toyama, Y, Matsumoto, M , Nakamura, M & Okano, H 2016, 'Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury', Stem cell reports, vol. 6, no. 1, pp. 1-8. https://doi.org/10.1016/j.stemcr.2015.11.013

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title = "Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury",

abstract = "Murine- and human-induced pluripotent stem cell-derived neural stem/progenitor cells (iPSC-NS/PCs) promote functional recovery following transplantation into the injured spinal cord in rodents and primates. Although remyelination of spared demyelinated axons is a critical mechanism in the regeneration of the injured spinal cord, human iPSC-NS/PCs predominantly differentiate into neurons both in vitro and in vivo. We therefore took advantage of our recently developed protocol to obtain human-induced pluripotent stem cell-derived oligodendrocyte precursor cell-enriched neural stem/progenitor cells and report the benefits of transplanting these cells in a spinal cord injury (SCI) model. We describe how this approach contributes to the robust remyelination of demyelinated axons and facilitates functional recovery after SCI.",

author = "Soya Kawabata and Morito Takano and Yuko Numasawa-Kuroiwa and Go Itakura and Yoshiomi Kobayashi and Yuichiro Nishiyama and Keiko Sugai and Soraya Nishimura and Hiroki Iwai and Miho Isoda and Shinsuke Shibata and Jun Kohyama and Akio Iwanami and Yoshiaki Toyama and Morio Matsumoto and Masaya Nakamura and Hideyuki Okano",

note = "Funding Information: We thank Prof. Shinya Yamanaka for the human iPSC clones (201B7). This work was supported by grants from the Japan Science and Technology-California Institute for Regenerative Medicine Collaborative Program ; Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (SPS) and the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT); and a grant for the Research Center Network for Realization of Regenerative Medicine from the A-MED to H.O. H.O. is a founding scientist and a paid SAB of San Bio, Co., Ltd. The other authors indicated no potential conflicts of interest. Publisher Copyright: {\textcopyright} 2016 The Authors.",

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doi = "10.1016/j.stemcr.2015.11.013",

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AU - Takano, Morito

AU - Numasawa-Kuroiwa, Yuko

AU - Itakura, Go

AU - Kobayashi, Yoshiomi

AU - Nishiyama, Yuichiro

AU - Sugai, Keiko

AU - Nishimura, Soraya

AU - Iwai, Hiroki

AU - Isoda, Miho

AU - Shibata, Shinsuke

AU - Kohyama, Jun

AU - Iwanami, Akio

AU - Toyama, Yoshiaki

AU - Matsumoto, Morio

AU - Nakamura, Masaya

AU - Okano, Hideyuki

N1 - Funding Information: We thank Prof. Shinya Yamanaka for the human iPSC clones (201B7). This work was supported by grants from the Japan Science and Technology-California Institute for Regenerative Medicine Collaborative Program ; Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (SPS) and the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT); and a grant for the Research Center Network for Realization of Regenerative Medicine from the A-MED to H.O. H.O. is a founding scientist and a paid SAB of San Bio, Co., Ltd. The other authors indicated no potential conflicts of interest. Publisher Copyright: © 2016 The Authors.

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Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury

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