Green marker for colonies of Bacillus subtilis

Mitsuhiro Itaya; Syed M. Shaheduzzaman; Kuniko Matsui; Akira Omori; Takashi Tsuji

doi:10.1271/bbb.65.579

Green marker for colonies of Bacillus subtilis

Mitsuhiro Itaya, Syed M. Shaheduzzaman, Kuniko Matsui, Akira Omori, Takashi Tsuji

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

Original language	English
Pages (from-to)	579-583
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	65
Issue number	3
DOIs	https://doi.org/10.1271/bbb.65.579
Publication status	Published - 2001 Mar
Externally published	Yes

Keywords

Bacillus subtilis
Fused protein
Green fluorescence protein
Multi-copy plasmid

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Green marker for colonies of Bacillus subtilis",

abstract = "A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.",

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doi = "10.1271/bbb.65.579",

language = "English",

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T1 - Green marker for colonies of Bacillus subtilis

AU - Itaya, Mitsuhiro

AU - Shaheduzzaman, Syed M.

AU - Matsui, Kuniko

AU - Omori, Akira

AU - Tsuji, Takashi

PY - 2001/3

Y1 - 2001/3

N2 - A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

AB - A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

KW - Bacillus subtilis

KW - Fused protein

KW - Green fluorescence protein

KW - Multi-copy plasmid

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DO - 10.1271/bbb.65.579

M3 - Article

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AN - SCOPUS:0035293122

SN - 0916-8451

VL - 65

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EP - 583

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 3

ER -

Green marker for colonies of Bacillus subtilis

Abstract

Keywords

ASJC Scopus subject areas

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