Green marker for colonies of Bacillus subtilis

Mitsuhiro Itaya, Syed M. Shaheduzzaman, Kuniko Matsui, Akira Omori, Takashi Tsuji

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

Original languageEnglish
Pages (from-to)579-583
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 2001 Mar
Externally publishedYes


  • Bacillus subtilis
  • Fused protein
  • Green fluorescence protein
  • Multi-copy plasmid

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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