Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar

S. A.M. Khalifa, E. Imai, Seiki Kobayashi, A. Haghighi, E. Hayakawa, T. Takeuchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


A growth-promoting factor (GPF) that promotes the growth of Entamoeba dispar under axenic culture conditions was found in fractions of mitochondria (Mt), hydrogenosomes (Hg) and chloroplasts (Cp) obtained from cells of six different protozoan, mammalian and plant species. We were able to extract the GPF from the Cp-rich leaf cells of a plant (spiderwort: Commelina communis L.) in an acetone-soluble fraction as a complex of chlorophyll with low molecular weight proteins (molecular weight [MW] approximately 4,600). We also found that on treatment with 0.6 % complexes of 2-mercapthoethonol (2ME), complexes of chlorophyll-a with iron-sulphur (Fe-S) proteins (e.g., ferredoxins [Fd] from spinach and Clostridium pasteurianum) and noncomplex rubredoxin (Rd) from C. pasteurianum have a growth-promoting effect on E. dispar. These findings suggest that E. dispar may lack a sufficient quantity of some essential components of Fe-S proteins, such as Fe-S center.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
Issue number1
Publication statusPublished - 2006 Mar
Externally publishedYes


  • Chloroplasts
  • Entamoeba dispar
  • Growth
  • Hydrogenosomes
  • Iron-sulphur protein
  • Mitochondria

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases


Dive into the research topics of 'Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar'. Together they form a unique fingerprint.

Cite this