Heat-shock protein 60 is required for blastema formation and maintenance during regeneration

Shinji Makino, Geoffrey G. Whitehead, Ching Ling Lien, Soo Kim, Payal Jhawar, Akane Kono, Yasushi Kawata, Mark T. Keating

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)


Zebrafish fin regeneration requires the formation and maintenance of blastema cells. Blastema cells are not derived from stem cells but behave as such, because they are slow-cycling and are thought to provide rapidly proliferating daughter cells that drive regenerative outgrowth. The molecular basis of blastema formation is not understood. Here, we show that heat-shock protein 60 (hsp60) is required for blastema formation and maintenance. We used a chemical mutagenesis screen to identify no blastema (nbl), a zebrafish mutant with an early fin regeneration defect. Fin regeneration failed in nbl due to defective blastema formation. nbl also failed to regenerate hearts. Positional cloning and mutational analyses revealed that nbl results from a V324E missense mutation in hsp60. This mutation reduced hsp60 function in binding and refolding denatured proteins. hsp60 expression is increased during formation of blastema cells, and dysfunction leads to mitochondrial defects and apoptosis in these cells. These data indicate that hsp60 is required for the formation and maintenance of regenerating tissue.

Original languageEnglish
Pages (from-to)14599-14604
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number41
Publication statusPublished - 2005 Oct 11
Externally publishedYes


  • Blastema
  • Genetics
  • Regeneration
  • Stress response
  • Zebrafish

ASJC Scopus subject areas

  • General


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