Heme oxygenase-1 inhibits cytokine production by activated mast cells

Yumiko Yasui, Mao Nakamura, Toshihiro Onda, Tomohiro Uehara, Saori Murata, Nobuaki Matsui, Nobuyuki Fukuishi, Reiko Akagi, Makoto Suematsu, Masaaki Akagi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Heme oxygenase-1 (HO-1) is thought to contribute to host defense reactions against various stresses. In addition, recent reports have suggested that HO-1 modulates immunocyte activation and functions. HO-1 suppresses mast cell degranulation, but whether HO-1 suppresses cytokine synthesis as well is not yet known. We examined whether rat HO-1 cDNA transfected rat basophilic leukemia (RBL)-2H3 cells have altered cytokine production in response to stimulation with anti-ovalbumin (OA) serum/OA compared to Mock transfected RBL-2H3 cells. HO-1 inhibited anti-OA serum/OA-induced IL-3 and TNF-α production. Inhibition of HO-1 activity by Zn (II) protoporphyrin IX, a specific HO-1 inhibitor, prevented the suppression of TNF-α production. The cytokine inhibition by HO-1 was associated with selective suppression of the DNA-binding activity of AP-1 transcription factors. The suppression of mast cell cytokine production by HO-1 may be an important aspect of the processes that lead to resolution of allergic inflammation.

Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 2007 Mar 9


  • AP-1
  • Heme oxygenase-1
  • IL-3
  • Mast cell
  • TNF-α

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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