Heme oxygenase-1 inhibits cytokine production by activated mast cells

Yumiko Yasui; Mao Nakamura; Toshihiro Onda; Tomohiro Uehara; Saori Murata; Nobuaki Matsui; Nobuyuki Fukuishi; Reiko Akagi; Makoto Suematsu; Masaaki Akagi

doi:10.1016/j.bbrc.2006.12.228

Heme oxygenase-1 inhibits cytokine production by activated mast cells

Yumiko Yasui, Mao Nakamura, Toshihiro Onda, Tomohiro Uehara, Saori Murata, Nobuaki Matsui, Nobuyuki Fukuishi, Reiko Akagi, Makoto Suematsu, Masaaki Akagi

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Heme oxygenase-1 (HO-1) is thought to contribute to host defense reactions against various stresses. In addition, recent reports have suggested that HO-1 modulates immunocyte activation and functions. HO-1 suppresses mast cell degranulation, but whether HO-1 suppresses cytokine synthesis as well is not yet known. We examined whether rat HO-1 cDNA transfected rat basophilic leukemia (RBL)-2H3 cells have altered cytokine production in response to stimulation with anti-ovalbumin (OA) serum/OA compared to Mock transfected RBL-2H3 cells. HO-1 inhibited anti-OA serum/OA-induced IL-3 and TNF-α production. Inhibition of HO-1 activity by Zn (II) protoporphyrin IX, a specific HO-1 inhibitor, prevented the suppression of TNF-α production. The cytokine inhibition by HO-1 was associated with selective suppression of the DNA-binding activity of AP-1 transcription factors. The suppression of mast cell cytokine production by HO-1 may be an important aspect of the processes that lead to resolution of allergic inflammation.

Original language	English
Pages (from-to)	485-490
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	354
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2006.12.228
Publication status	Published - 2007 Mar 9

Keywords

AP-1
Heme oxygenase-1
IL-3
Mast cell
TNF-α

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.12.228

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title = "Heme oxygenase-1 inhibits cytokine production by activated mast cells",

abstract = "Heme oxygenase-1 (HO-1) is thought to contribute to host defense reactions against various stresses. In addition, recent reports have suggested that HO-1 modulates immunocyte activation and functions. HO-1 suppresses mast cell degranulation, but whether HO-1 suppresses cytokine synthesis as well is not yet known. We examined whether rat HO-1 cDNA transfected rat basophilic leukemia (RBL)-2H3 cells have altered cytokine production in response to stimulation with anti-ovalbumin (OA) serum/OA compared to Mock transfected RBL-2H3 cells. HO-1 inhibited anti-OA serum/OA-induced IL-3 and TNF-α production. Inhibition of HO-1 activity by Zn (II) protoporphyrin IX, a specific HO-1 inhibitor, prevented the suppression of TNF-α production. The cytokine inhibition by HO-1 was associated with selective suppression of the DNA-binding activity of AP-1 transcription factors. The suppression of mast cell cytokine production by HO-1 may be an important aspect of the processes that lead to resolution of allergic inflammation.",

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author = "Yumiko Yasui and Mao Nakamura and Toshihiro Onda and Tomohiro Uehara and Saori Murata and Nobuaki Matsui and Nobuyuki Fukuishi and Reiko Akagi and Makoto Suematsu and Masaaki Akagi",

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AU - Onda, Toshihiro

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AU - Murata, Saori

AU - Matsui, Nobuaki

AU - Fukuishi, Nobuyuki

AU - Akagi, Reiko

AU - Suematsu, Makoto

AU - Akagi, Masaaki

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N2 - Heme oxygenase-1 (HO-1) is thought to contribute to host defense reactions against various stresses. In addition, recent reports have suggested that HO-1 modulates immunocyte activation and functions. HO-1 suppresses mast cell degranulation, but whether HO-1 suppresses cytokine synthesis as well is not yet known. We examined whether rat HO-1 cDNA transfected rat basophilic leukemia (RBL)-2H3 cells have altered cytokine production in response to stimulation with anti-ovalbumin (OA) serum/OA compared to Mock transfected RBL-2H3 cells. HO-1 inhibited anti-OA serum/OA-induced IL-3 and TNF-α production. Inhibition of HO-1 activity by Zn (II) protoporphyrin IX, a specific HO-1 inhibitor, prevented the suppression of TNF-α production. The cytokine inhibition by HO-1 was associated with selective suppression of the DNA-binding activity of AP-1 transcription factors. The suppression of mast cell cytokine production by HO-1 may be an important aspect of the processes that lead to resolution of allergic inflammation.

AB - Heme oxygenase-1 (HO-1) is thought to contribute to host defense reactions against various stresses. In addition, recent reports have suggested that HO-1 modulates immunocyte activation and functions. HO-1 suppresses mast cell degranulation, but whether HO-1 suppresses cytokine synthesis as well is not yet known. We examined whether rat HO-1 cDNA transfected rat basophilic leukemia (RBL)-2H3 cells have altered cytokine production in response to stimulation with anti-ovalbumin (OA) serum/OA compared to Mock transfected RBL-2H3 cells. HO-1 inhibited anti-OA serum/OA-induced IL-3 and TNF-α production. Inhibition of HO-1 activity by Zn (II) protoporphyrin IX, a specific HO-1 inhibitor, prevented the suppression of TNF-α production. The cytokine inhibition by HO-1 was associated with selective suppression of the DNA-binding activity of AP-1 transcription factors. The suppression of mast cell cytokine production by HO-1 may be an important aspect of the processes that lead to resolution of allergic inflammation.

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KW - Mast cell

KW - TNF-α

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Heme oxygenase-1 inhibits cytokine production by activated mast cells

Abstract

Keywords

ASJC Scopus subject areas

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