Hemicholinium-3-resistant choline uptake system linked to acetylcholine synthesis in the rat hippocampus

Takeshi Suzuki; Yuko Kashima; Koichiro Kawashima

doi:10.1016/0304-3940(89)90039-6

Hemicholinium-3-resistant choline uptake system linked to acetylcholine synthesis in the rat hippocampus

Takeshi Suzuki, Yuko Kashima, Koichiro Kawashima

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Extracellular choline-dependency of acetylcholine (ACh) synthesis was examined in rat hippocampal slices. In the presence of hemicholinium-3 (HC-3, 5 μM), extracellular choline-dependent ACh synthesis appeared to consist of two different components. The first component (saturated at up to 50 μM choline) was dependent on the HC-3-resistant choline uptake system having a low capacity for choline in comparison with the high-affinity choline uptake system (HACU). The second component, observed in the presence of more than 50 μM choline, was considered due to competitive inhibition of HACU by HC-3. HACU-dependent ACh synthesis seemed to be saturated in the presence of up to 2 μM choline. These results indicate that both the HACU and HC-3-resistant choline uptake systems are linked to ACh synthesis in the hippocampus.

Original language	English
Pages (from-to)	211-214
Number of pages	4
Journal	Neuroscience Letters
Volume	105
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(89)90039-6
Publication status	Published - 1989 Oct 23

Keywords

Acetylcholine synthesis
Choline
Hemicholinium-3
High-affinity choline uptake system
Hippocampus
Radioimmunoassay

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(89)90039-6

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title = "Hemicholinium-3-resistant choline uptake system linked to acetylcholine synthesis in the rat hippocampus",

abstract = "Extracellular choline-dependency of acetylcholine (ACh) synthesis was examined in rat hippocampal slices. In the presence of hemicholinium-3 (HC-3, 5 μM), extracellular choline-dependent ACh synthesis appeared to consist of two different components. The first component (saturated at up to 50 μM choline) was dependent on the HC-3-resistant choline uptake system having a low capacity for choline in comparison with the high-affinity choline uptake system (HACU). The second component, observed in the presence of more than 50 μM choline, was considered due to competitive inhibition of HACU by HC-3. HACU-dependent ACh synthesis seemed to be saturated in the presence of up to 2 μM choline. These results indicate that both the HACU and HC-3-resistant choline uptake systems are linked to ACh synthesis in the hippocampus.",

keywords = "Acetylcholine synthesis, Choline, Hemicholinium-3, High-affinity choline uptake system, Hippocampus, Radioimmunoassay",

author = "Takeshi Suzuki and Yuko Kashima and Koichiro Kawashima",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science and Culture, Japan (63771971).",

year = "1989",

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doi = "10.1016/0304-3940(89)90039-6",

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pages = "211--214",

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AU - Suzuki, Takeshi

AU - Kashima, Yuko

AU - Kawashima, Koichiro

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science and Culture, Japan (63771971).

PY - 1989/10/23

Y1 - 1989/10/23

N2 - Extracellular choline-dependency of acetylcholine (ACh) synthesis was examined in rat hippocampal slices. In the presence of hemicholinium-3 (HC-3, 5 μM), extracellular choline-dependent ACh synthesis appeared to consist of two different components. The first component (saturated at up to 50 μM choline) was dependent on the HC-3-resistant choline uptake system having a low capacity for choline in comparison with the high-affinity choline uptake system (HACU). The second component, observed in the presence of more than 50 μM choline, was considered due to competitive inhibition of HACU by HC-3. HACU-dependent ACh synthesis seemed to be saturated in the presence of up to 2 μM choline. These results indicate that both the HACU and HC-3-resistant choline uptake systems are linked to ACh synthesis in the hippocampus.

AB - Extracellular choline-dependency of acetylcholine (ACh) synthesis was examined in rat hippocampal slices. In the presence of hemicholinium-3 (HC-3, 5 μM), extracellular choline-dependent ACh synthesis appeared to consist of two different components. The first component (saturated at up to 50 μM choline) was dependent on the HC-3-resistant choline uptake system having a low capacity for choline in comparison with the high-affinity choline uptake system (HACU). The second component, observed in the presence of more than 50 μM choline, was considered due to competitive inhibition of HACU by HC-3. HACU-dependent ACh synthesis seemed to be saturated in the presence of up to 2 μM choline. These results indicate that both the HACU and HC-3-resistant choline uptake systems are linked to ACh synthesis in the hippocampus.

KW - Acetylcholine synthesis

KW - Choline

KW - Hemicholinium-3

KW - High-affinity choline uptake system

KW - Hippocampus

KW - Radioimmunoassay

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AN - SCOPUS:0024455687

SN - 0304-3940

VL - 105

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Hemicholinium-3-resistant choline uptake system linked to acetylcholine synthesis in the rat hippocampus

Abstract

Keywords

ASJC Scopus subject areas

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