TY - JOUR
T1 - Hepatocyte growth factor promotes proliferation and neuronal differentiation of neural stem cells from mouse embryos
AU - Kokuzawa, Jouji
AU - Yoshimura, Shinichi
AU - Kitajima, Hideomi
AU - Shinoda, Jun
AU - Kaku, Yasuhiko
AU - Iwama, Toru
AU - Morishita, Ryuichi
AU - Shimazaki, Takuya
AU - Okano, Hideyuki
AU - Kunisada, Takahiro
AU - Sakai, Noboru
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Hepatocyte growth factor (HGF), originally cloned as a hepatocyte mitogen, has recently been reported to exhibit neurotrophic activity in addition to being expressed in different parts of the nervous system. At present, the effects of HGF on neural stem cells (NSCs) are not known. In this study, we first report the promoting effect of HGF on the proliferation of neurospheres and neuronal differentiation of NSCs. Medium containing only HGF was capable of inducing neurosphere formation. Addition of HGF to medium containing fibroblast growth factor 2 or epidermal growth factor increased both the size and number of newly formed neurospheres. More neurons were also obtained when HGF was added in differentiation medium. In contrast, neurosphere numbers were reduced after repeated subculture by mechanical dissociation, suggesting that HGF-formed neurospheres comprised predominantly progenitor cells committed to neuronal or glial lines. Together, these results suggest that HGF promotes proliferation of neurospheres and neuronal differentiation of NSCs derived from mouse embyos.
AB - Hepatocyte growth factor (HGF), originally cloned as a hepatocyte mitogen, has recently been reported to exhibit neurotrophic activity in addition to being expressed in different parts of the nervous system. At present, the effects of HGF on neural stem cells (NSCs) are not known. In this study, we first report the promoting effect of HGF on the proliferation of neurospheres and neuronal differentiation of NSCs. Medium containing only HGF was capable of inducing neurosphere formation. Addition of HGF to medium containing fibroblast growth factor 2 or epidermal growth factor increased both the size and number of newly formed neurospheres. More neurons were also obtained when HGF was added in differentiation medium. In contrast, neurosphere numbers were reduced after repeated subculture by mechanical dissociation, suggesting that HGF-formed neurospheres comprised predominantly progenitor cells committed to neuronal or glial lines. Together, these results suggest that HGF promotes proliferation of neurospheres and neuronal differentiation of NSCs derived from mouse embyos.
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U2 - 10.1016/S1044-7431(03)00160-X
DO - 10.1016/S1044-7431(03)00160-X
M3 - Article
C2 - 14550779
AN - SCOPUS:10744233440
SN - 1044-7431
VL - 24
SP - 190
EP - 197
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -