High-resolution microfluidic paper-based analytical devices for sub-microliter sample analysis

Keisuke Tenda, Riki Ota, Kentaro Yamada, Terence G. Henares, Koji Suzuki, Daniel Citterio

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)


This work demonstrates the fabrication of microfluidic paper-based analytical devices (μPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures patterned in filter paper. This has been achieved by replacing the hot plate heating method conventionally used to melt printed wax features into paper by simple hot lamination. This patterning technique, in combination with the consideration of device geometry and the influence of cellulose fiber direction in filter paper, led to a model μPAD design with four microfluidic channels that can be filled with as low as 0.5 μL of liquid. Finally, the application to a colorimetric model assay targeting total protein concentrations is shown. Calibration curves for human serum albumin (HSA) were recorded from sub-microliter samples (0.8 μL), with tolerance against ±0.1 μL variations in the applied liquid volume.

Original languageEnglish
Article number80
Issue number5
Publication statusPublished - 2016


  • Colorimetry
  • Inkjet printing
  • Protein assay
  • Wax printing
  • μPAD

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering


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