TY - GEN
T1 - HIGH-SENSITIVITY DETECTION BY AN INTERFACE OF MASS SPECTROMETRY UTILIZING FEMTOLITER-DROPLET NANOFLUIDICS
AU - Kazoe, Yutaka
AU - Takagi, Yuto
AU - Morikawa, Kyojiro
AU - Kitamori, Takehiko
N1 - Funding Information:
The authors gratefully acknowledge the financial support of JST CREST (JPMJCR14G1).
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - We established an interface of mass spectrometry based on nanofluidics. After applying a high voltage, liquid sample was converted to fL-droplets by gas-phase flow focusing in micro/nanochannels, and they were ejected into a mass spectrometer (MS) with 100% efficiency by trajectory control, which has been difficult for conventional MS interface by electrospray ionization (ESI) with sample dispersion. The injected droplets were evaporated to generate gas-phase analyte ions by heat transfer. We realized 290 times higher MS sensitivity compared to conventional ESI for the first time. The method will greatly contribute to the state-of-the-art analyses such as single-cell proteomics.
AB - We established an interface of mass spectrometry based on nanofluidics. After applying a high voltage, liquid sample was converted to fL-droplets by gas-phase flow focusing in micro/nanochannels, and they were ejected into a mass spectrometer (MS) with 100% efficiency by trajectory control, which has been difficult for conventional MS interface by electrospray ionization (ESI) with sample dispersion. The injected droplets were evaporated to generate gas-phase analyte ions by heat transfer. We realized 290 times higher MS sensitivity compared to conventional ESI for the first time. The method will greatly contribute to the state-of-the-art analyses such as single-cell proteomics.
KW - Droplet
KW - Femtoliter
KW - Mass spectrometry
KW - Nanofluidics
UR - http://www.scopus.com/inward/record.url?scp=85136952433&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:85136952433
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 59
EP - 60
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -