High-speed super-multiplex imaging of brain tissue

Robert Oda, Jingwen Shou, Keiko Karasawa, Mutsuo Nuriya, Masato Yasui, Yasuyuki Ozeki

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Cells within the brain are highly organized and coordinate complex processes with each other. The ability to simultaneously visualize the organization and interactions of cells and molecules within brain tissue remains an important issue to understand the brain comprehensively. Stimulated Raman scattering (SRS) and fluorescence, two powerful imaging modalities, can provide complementary molecular contrasts within cells and tissue samples. Here, we present a high-speed super-multiplex imaging platform that combines SRS microscopy with confocal fluorescence microscopy to perform rapid 7-color brain imaging. We show simultaneous imaging of cellular components within the brain such as astrocytes, axons, and blood vessels while also showing organelles such as the nucleus and actin cytoskeleton. Also, we demonstrate the ability to take depth-resolved images that elucidate the three-dimensional organization of diverse components within brain tissue. This platform can be adapted to explore various processes within brain tissue that can reveal critical information about the brain and how it is affected by diseases, which leads toward a deeper understanding of disease progression and potentially the development of therapeutic options for brain diseases.

Original languageEnglish
Title of host publicationAdvanced Chemical Microscopy for Life Science and Translational Medicine 2021
EditorsJi-Xin Cheng, Wei Min, Garth J. Simpson
ISBN (Electronic)9781510641471
Publication statusPublished - 2021
EventAdvanced Chemical Microscopy for Life Science and Translational Medicine 2021 - Virtual, Online, United States
Duration: 2021 Mar 62021 Mar 11

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceAdvanced Chemical Microscopy for Life Science and Translational Medicine 2021
Country/TerritoryUnited States
CityVirtual, Online


  • Neuroimaging
  • Stimulated Raman scattering microscopy
  • Super multiplex

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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