TY - JOUR
T1 - Hippocampal memory traces are differentially modulated by experience, time, and adult neurogenesis
AU - Denny, Christine A.
AU - Kheirbek, Mazen A.
AU - Alba, Eva L.
AU - Tanaka, Kenji F.
AU - Brachman, Rebecca A.
AU - Laughman, Kimberly B.
AU - Tomm, Nicole K.
AU - Turi, Gergely F.
AU - Losonczy, Attila
AU - Hen, René
N1 - Funding Information:
C.A.D. was supported by grants from the National Institutes of Research (NIH) (F31MH084529-01, T32 MH015174-36, and T32 GM008798-8). M.A.K. was supported by a NIH grant (K01MH099371-01), a U.S. National Alliance for Research in Schizophrenia and Depression (NARSAD) Young Investigator Award, and a Sackler Institute Award. K.F.T. was supported by a NARSAD 2008 Young Investigator Award. R.H. was supported by a NARSAD, the New York Stem Cell Initiative, and a NIH grant (R01 MH068542). We thank members of the Hen laboratory, R. Axel, S.A. Siegelbaum, S. Fusi, A.A. Fenton, K. Martinowich, and J.V. Kupferman for insightful comments on this project and manuscript and M. Thomsen, A. Jonathan, and M.R. Drew for technical assistance early in this project. Cognitive and behavioral phenotyping experiments utilized the facilities of the Rodent Models Neurobehavioral Analysis Core of the Lieber Center for Schizophrenia Research at Columbia University and the New York State Psychiatric Institute (NYSPI).
PY - 2014/7/2
Y1 - 2014/7/2
N2 - Memory traces are believed to be ensembles of cells used to store memories. To visualize memory traces, we created a transgenic line that allows for the comparison between cells activated during encoding and expression of a memory. Mice re-exposed to a fear-inducing context froze more and had a greater percentage of reactivated cells in the dentate gyrus (DG) and CA3 than mice exposed to a novel context. Over time, these differences disappeared, in keeping with the observation that memories become generalized. Optogenetically silencing DG or CA3 cells that were recruited during encoding of a fear-inducing context prevented expression of the corresponding memory. Mice with reduced neurogenesis displayed less contextual memory and less reactivation in CA3but, surprisingly, normal reactivation in the DG. These studies suggest that distinct memory traces are located in the DG and in CA3 but that the strength of the memory is related to reactivation in CA3.
AB - Memory traces are believed to be ensembles of cells used to store memories. To visualize memory traces, we created a transgenic line that allows for the comparison between cells activated during encoding and expression of a memory. Mice re-exposed to a fear-inducing context froze more and had a greater percentage of reactivated cells in the dentate gyrus (DG) and CA3 than mice exposed to a novel context. Over time, these differences disappeared, in keeping with the observation that memories become generalized. Optogenetically silencing DG or CA3 cells that were recruited during encoding of a fear-inducing context prevented expression of the corresponding memory. Mice with reduced neurogenesis displayed less contextual memory and less reactivation in CA3but, surprisingly, normal reactivation in the DG. These studies suggest that distinct memory traces are located in the DG and in CA3 but that the strength of the memory is related to reactivation in CA3.
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U2 - 10.1016/j.neuron.2014.05.018
DO - 10.1016/j.neuron.2014.05.018
M3 - Article
C2 - 24991962
AN - SCOPUS:84903584973
SN - 0896-6273
VL - 83
SP - 189
EP - 201
JO - Neuron
JF - Neuron
IS - 1
ER -