HLA class I and class II expression of pulmonary adenocarcinoma cells and the influence of interferon gamma

Tokujiro Yano, Yasuro Fukuyama, Hideki Yokoyama, Shinji Kuninaka, Hiroshi Asoh, Yasaburo Katsuda, Yukito Ichinose

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Background: The clinico-biological significance of HLA (both class I antigen and class II one) expressed on tumor cells still remains controversial. Methods: Tumor cells were freshly separated from 33 surgical specimens of pulmonary adenocarcinoma. The tumor cells were incubated for 24 h in the presence or absence of IFN-γ (130 International Units/ml). After incubation, the cells were cytocentrifuged onto glass slides and immunostained with either an anti-HLA class I (A, B, C) monoclonal antibody or anti-HLA class II (DR) one. Results: In 22 of 33 cases (66.7%), the HLA class I were individually expressed by more than 60% of tumor cells while so were the HLA class II in 15 (45.4%). No significant correlation was observed between the HLA class I expression and the HLA class II one. The proportion of HLA class I-positive tumor cells correlated with neither the grade of histological differentiation nor the stage of disease. In contrast, the proportion of HLA class II-positive tumor cells correlated with both the grade of histological differentiation and the stage. In most cases, IFN-γ was found to increase the proportion of class II-positive tumor cells as well as that of class I-positive cells. Conclusions: The above findings thus suggested that the HLA class II expression might therefore represent a manifestation of cellular differentiation and that IFN-γ may, as a result, have the potential to differentiate cancer cells. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalLung Cancer
Issue number3
Publication statusPublished - 1998 Jun


  • Differentiation
  • HLA class I
  • HLA class II
  • IFN-γ
  • Immunocytochemistry
  • Pulmonary adenocarcinoma
  • Stage of the disease

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research


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