hnRNP-U enhances the expression of specific genes by stabilizing mRNA

Masato Yugami; Yasuaki Kabe; Yuki Yamaguchi; Tadashi Wada; Hiroshi Handa

doi:10.1016/j.febslet.2006.11.062

hnRNP-U enhances the expression of specific genes by stabilizing mRNA

Masato Yugami
, Yasuaki Kabe
, Yuki Yamaguchi
, Tadashi Wada
, Hiroshi Handa

Research output: Contribution to journal › Article › peer-review

73 Citations (Scopus)

Abstract

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements. However, its role in the regulation of gene expression is as yet poorly understood. In the present study, we show that hnRNP-U specifically enhances the expression of tumor necrosis factor α mRNA by increasing its stability, possibly through binding to the 3′ untranslated region. We also show that hnRNP-U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER3, NHLH2, and ZFY, by binding to and stabilizing these mRNAs. These results suggest that hnRNP-U enhances the expression of specific genes by regulating mRNA stability.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	1
DOIs	https://doi.org/10.1016/j.febslet.2006.11.062
Publication status	Published - 2007 Jan 9
Externally published	Yes

Keywords

3′ Untranslated region
Gene expression
Heterogeneous ribonucleoprotein
Tumor necrosis factor

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.11.062

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title = "hnRNP-U enhances the expression of specific genes by stabilizing mRNA",

abstract = "Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements. However, its role in the regulation of gene expression is as yet poorly understood. In the present study, we show that hnRNP-U specifically enhances the expression of tumor necrosis factor α mRNA by increasing its stability, possibly through binding to the 3′ untranslated region. We also show that hnRNP-U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER3, NHLH2, and ZFY, by binding to and stabilizing these mRNAs. These results suggest that hnRNP-U enhances the expression of specific genes by regulating mRNA stability.",

keywords = "3′ Untranslated region, Gene expression, Heterogeneous ribonucleoprotein, Tumor necrosis factor",

author = "Masato Yugami and Yasuaki Kabe and Yuki Yamaguchi and Tadashi Wada and Hiroshi Handa",

note = "Funding Information: We thank Yohei Amano and Masafumi Suzuki for technical assistance. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas, the Special Coordination Funds for Promoting Science and Technology of the Ministry of Education and the Grant of the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. This work was also supported by a grant from the New Energy and Industrial Technology Development Organization, Japan to H.H. M.Y. is a JSPS research fellow. ",

year = "2007",

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doi = "10.1016/j.febslet.2006.11.062",

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AU - Yugami, Masato

AU - Kabe, Yasuaki

AU - Yamaguchi, Yuki

AU - Wada, Tadashi

AU - Handa, Hiroshi

N1 - Funding Information: We thank Yohei Amano and Masafumi Suzuki for technical assistance. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas, the Special Coordination Funds for Promoting Science and Technology of the Ministry of Education and the Grant of the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. This work was also supported by a grant from the New Energy and Industrial Technology Development Organization, Japan to H.H. M.Y. is a JSPS research fellow.

PY - 2007/1/9

Y1 - 2007/1/9

N2 - Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements. However, its role in the regulation of gene expression is as yet poorly understood. In the present study, we show that hnRNP-U specifically enhances the expression of tumor necrosis factor α mRNA by increasing its stability, possibly through binding to the 3′ untranslated region. We also show that hnRNP-U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER3, NHLH2, and ZFY, by binding to and stabilizing these mRNAs. These results suggest that hnRNP-U enhances the expression of specific genes by regulating mRNA stability.

AB - Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements. However, its role in the regulation of gene expression is as yet poorly understood. In the present study, we show that hnRNP-U specifically enhances the expression of tumor necrosis factor α mRNA by increasing its stability, possibly through binding to the 3′ untranslated region. We also show that hnRNP-U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER3, NHLH2, and ZFY, by binding to and stabilizing these mRNAs. These results suggest that hnRNP-U enhances the expression of specific genes by regulating mRNA stability.

KW - 3′ Untranslated region

KW - Gene expression

KW - Heterogeneous ribonucleoprotein

KW - Tumor necrosis factor

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hnRNP-U enhances the expression of specific genes by stabilizing mRNA

Abstract

Keywords

ASJC Scopus subject areas

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