TY - JOUR
T1 - Homozygous deletions and chromosome amplifications in human lung carcinomas revealed by single nucleotide polymorphism array analysis
AU - Zhao, Xiaojun
AU - Weir, Barbara A.
AU - LaFramboise, Thomas
AU - Lin, Ming
AU - Beroukhim, Rameen
AU - Garraway, Levi
AU - Beheshti, Javad
AU - Lee, Jeffrey C.
AU - Naoki, Katsuhiko
AU - Richards, William G.
AU - Sugarbaker, David
AU - Chen, Fei
AU - Rubin, Mark A.
AU - Jänne, Pasi A.
AU - Girard, Luc
AU - Minna, John
AU - Christiani, David
AU - Li, Cheng
AU - Sellers, William R.
AU - Meyerson, Matthew
PY - 2005/7/1
Y1 - 2005/7/1
N2 - Genome-wide copy number changes were analyzed in 70 primary human lung carcinoma specimens and 31 cell lines derived from human lung carcinomas, with high-density arrays representing ∼115,000 single nucleotide polymorphism loci. In addition to previously characterized loci, two regions of homozygous deletion were found, one near the PTPRD locus on chromosome segment 9p23 in four samples representing both small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC) and the second on chromosome segment 3q25 in one sample each of NSCLC and SCLC. High-level amplifications were identified within chromosome segment 8q12-13 in two SCLC specimens, 12p11 in two NSCLC specimens and 22q11 in four NSCLC specimens. Systematic copy number analysis of tyrosine kinase genes identified high-level amplification of EGFR in three NSCLC specimens, FGFR1 in two specimens and ERBB2 and MET in one specimen each. EGFR amplification was shown to be independent of kinase domain mutational status.
AB - Genome-wide copy number changes were analyzed in 70 primary human lung carcinoma specimens and 31 cell lines derived from human lung carcinomas, with high-density arrays representing ∼115,000 single nucleotide polymorphism loci. In addition to previously characterized loci, two regions of homozygous deletion were found, one near the PTPRD locus on chromosome segment 9p23 in four samples representing both small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC) and the second on chromosome segment 3q25 in one sample each of NSCLC and SCLC. High-level amplifications were identified within chromosome segment 8q12-13 in two SCLC specimens, 12p11 in two NSCLC specimens and 22q11 in four NSCLC specimens. Systematic copy number analysis of tyrosine kinase genes identified high-level amplification of EGFR in three NSCLC specimens, FGFR1 in two specimens and ERBB2 and MET in one specimen each. EGFR amplification was shown to be independent of kinase domain mutational status.
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U2 - 10.1158/0008-5472.CAN-04-4603
DO - 10.1158/0008-5472.CAN-04-4603
M3 - Article
C2 - 15994928
AN - SCOPUS:21344437734
SN - 0008-5472
VL - 65
SP - 5561
EP - 5570
JO - Cancer Research
JF - Cancer Research
IS - 13
ER -