HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA

Hiroshi Kanamori, Noboru Suzuki, Haruhiko Siomi, Tetsuya Nosaka, Akihiko Sato, Hisataka Sabe, Masakazu Hatanaka, Tasuku Honjo

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Expression of the pX gene products (p40tax, p27rex and p21X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces expression of the interleukin-2 receptor a chain (IL-2Rα) on infected T cells. Comparison of IL-2Rα promoter activities has revealed that the transcriptional activation of the promoter alone cannot explain the large numbers of IL-2Rα expressed on HTLV-1 infected cells. We found that the rates of the IL-2Rα mRNA degradation were greatly reduced in pX-positive cells as compared with pX-negative cells. Simultaneous transfection of the expression vector plasmid containing IL-2Rα cDNA and similar plasmids containing various pX sequences showed that p27rex elongated the half life of IL-2Rα mRNA. As p27rex did not affect the transport of the IL-2Rα mRNA from nucleus to cytoplasm, prolongation of the IL-2Rα mRNA half life by p27rex is ascribed to stabilization of the mRNA. Experiments using deletion mutants and chimeric constructs of the IL-2Rα cDNA demonstrated that the coding sequence but not the 5′ or 3′ untranslated region of the IL-2Rα mRNA sequence is responsible for its protection by p27rex.

Original languageEnglish
Pages (from-to)4161-4166
Number of pages6
JournalEMBO Journal
Volume9
Issue number12
Publication statusPublished - 1990
Externally publishedYes

Keywords

  • Coding sequence
  • Interluekin-2 receptor α chain
  • Mrna regulation
  • P27

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology
  • Molecular Biology
  • General Neuroscience

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