Hydrogen peroxide enhances phagocytic activity of ameboid microglia

Hidetaka Takeda, Minoru Tomita, Norio Tanahashi, Masahiro Kobari, Masako Yokoyama, Masaki Takao, Daisuke Ito, Yasuo Fukuuchi

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Microglia are considered to serve as a guardian of the brain. In achieving this task, they have been observed to transform into a reactive form and then an ameboid form. Several substances are implicated in the control of such behavior. We examined the effect of hydrogen peroxide on cultured microglia of ameboid form obtained from the fetal rat brain employing video-enhanced contrast-differential interference contrast microscopy. Microglia harvested from the culture bottle were observed to float in a spherical shape with abundant filopodia on the surface. However, on coming into contact with the glass surface of a cover slip, they immediately transformed into an ameboid form. The microglia spread themselves out, surrounded by thin transparent lammellipodia, which would not be clearly observable by either light microscopy or electron microscopy. In a concentration-dependent fashion, 10-3-10-1 M hydrogen peroxide solution enhanced the ruffling process of the lamellipodia and formation of vesicles (phagosomes), which displayed a typical phagocytotic form. It is concluded that an increase in free radicals in ischemic tissue tends to facilitate the phagocytosis of ameboid microglia as macrophages.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalNeuroscience Letters
Issue number1
Publication statusPublished - 1998 Jan 2


  • Filopodia
  • Lamellipodia
  • Microglia
  • Phagosome
  • Ruffling
  • Vacuolization
  • Video- enhanced contrast-differential interference contrast microscopy

ASJC Scopus subject areas

  • Neuroscience(all)


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