TY - JOUR
T1 - Hydrogen-producing milk to prevent reduction in tear stability in persons using visual display terminals
AU - Kawashima, Motoko
AU - Tsuno, Saki
AU - Matsumoto, Mitsuharu
AU - Tsubota, Kazuo
N1 - Funding Information:
Kyodo Milk Industry Co. Ltd.(Tokyo, Japan) funded this research. The funder had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.M. Kawashima has nothing to disclose. S. Tsuno and M. Matsumoto are employees of Kyodo Milk Industry Co. Ltd.(Tokyo, Japan), which funded this research and supplies commercial products (H2-producing milk) related to the content of this study. Tsubota Laboratory, Inc. has a royalty right for the commercial product in this study (H2-producing milk).
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/10
Y1 - 2019/10
N2 - Purpose: To examine the effect of hydrogen (H2)-producing milk, which induces H2 production by intestinal microbiota, on the prevention of dry eye disease. Methods: A randomized, double-blind, placebo-controlled, parallel-group comparative study was conducted in humans. Of 118 candidates aged 20–60 years who were using visual display terminals daily, 54 subjects (mean age, 42.4 years) with mild-to-moderate dry eye symptoms, who were not dry eye patients based on the 2006 Japanese Diagnostic Criteria for Dry Eye, were included in the study. Subjects were assigned to the H2-producing milk group or the placebo group. They were instructed to ingest the assigned test beverage once per day for three consecutive weeks. Breath H2 concentration, fluorescein tear film breakup time (fTBUT), Schirmer's test, 8-OHdG concentration in tears, reported ocular symptoms using the dry eye-related QOL score questionnaire, and the visual analogue scale were assessed at weeks 0 and 3. Results: Change in fTBUT in the H2-producing milk group was significantly greater than that in the placebo group. A significant decline in fTBUT was observed in the placebo group (p = 0.04), but not the H2-producing group (p = 0.26). This phenomenon was strongly observed in females. We found a positive correlation between the change in fTBUT and net H2 production, and a negative correlation between the change in 8-OHdG concentration and net H2 production in young females. Conclusions: H2-producing milk appeared to retard the decline in tear stability and may prevent short fTBUT-type dry eye by decreasing oxidative stress in the lacrimal functional unit.
AB - Purpose: To examine the effect of hydrogen (H2)-producing milk, which induces H2 production by intestinal microbiota, on the prevention of dry eye disease. Methods: A randomized, double-blind, placebo-controlled, parallel-group comparative study was conducted in humans. Of 118 candidates aged 20–60 years who were using visual display terminals daily, 54 subjects (mean age, 42.4 years) with mild-to-moderate dry eye symptoms, who were not dry eye patients based on the 2006 Japanese Diagnostic Criteria for Dry Eye, were included in the study. Subjects were assigned to the H2-producing milk group or the placebo group. They were instructed to ingest the assigned test beverage once per day for three consecutive weeks. Breath H2 concentration, fluorescein tear film breakup time (fTBUT), Schirmer's test, 8-OHdG concentration in tears, reported ocular symptoms using the dry eye-related QOL score questionnaire, and the visual analogue scale were assessed at weeks 0 and 3. Results: Change in fTBUT in the H2-producing milk group was significantly greater than that in the placebo group. A significant decline in fTBUT was observed in the placebo group (p = 0.04), but not the H2-producing group (p = 0.26). This phenomenon was strongly observed in females. We found a positive correlation between the change in fTBUT and net H2 production, and a negative correlation between the change in 8-OHdG concentration and net H2 production in young females. Conclusions: H2-producing milk appeared to retard the decline in tear stability and may prevent short fTBUT-type dry eye by decreasing oxidative stress in the lacrimal functional unit.
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U2 - 10.1016/j.jtos.2019.07.008
DO - 10.1016/j.jtos.2019.07.008
M3 - Article
C2 - 31352083
AN - SCOPUS:85075090304
SN - 1542-0124
VL - 17
SP - 714
EP - 721
JO - Ocular Surface
JF - Ocular Surface
IS - 4
ER -