Hypermethylation in the p16 promoter region in the carcinogenesis of endometrial cancer in Japanese patients

Megumi Yanokura, Kouji Banno, Nobuyuki Susumu, Makiko Kawaguchi, Yoshiko Kuwabara, Katsumi Tsukazaki, Daisuke Aoki

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Background: p16 has been reported to disappear by hypermethylation in various cancers. However, the relationship between the frequency of hypermethylation of p16 and the mechanism of its inactivation has not been completely elucidated in endometrial cancer. Materials and Methods: Hypermethylation in the promoter region of the p16 gene and the expression of the p16 protein in 51 specimens, including 8 endometrial cancer cell lines, 7 normal endometrial tissues, 12 atypical endometrial hyperplasia tissues and 32 endometrial cancer tissues were analyzed. Results: Five out of 8 endometrial cancer cell lines showed hypermethylation with high frequency, although only 1 showed loss of gene expression. However, no endometrial cancer tissue of the 32 specimens showed hypermethylation. Furthermore, loss of expression was immunohistochemically observed in 3 out of the 20 specimens. Conclusion: These results suggest that hypermethylation of p16 rarely occurs and, thus, has no significant effect on the carcinogenesis of endometrial cancer in Japanese patients.

Original languageEnglish
Pages (from-to)851-856
Number of pages6
JournalAnticancer research
Issue number2 A
Publication statusPublished - 2006 Mar 1


  • Endometrial cancer
  • Hypermethylation
  • p16

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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