Identification and characterization of the high-affinity choline transporter

Takashi Okuda; Tatsuya Haga; Yoshikatsu Kanai; Hitoshi Endou; Takeshi Ishihara; Isao Katsura

doi:10.1038/72059

Identification and characterization of the high-affinity choline transporter

Takashi Okuda, Tatsuya Haga, Yoshikatsu Kanai, Hitoshi Endou, Takeshi Ishihara, Isao Katsura

Research output: Contribution to journal › Article › peer-review

279 Citations (Scopus)

Abstract

In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na⁺-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

Original language	English
Pages (from-to)	120-125
Number of pages	6
Journal	Nature Neuroscience
Volume	3
Issue number	2
DOIs	https://doi.org/10.1038/72059
Publication status	Published - 2000 Feb
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)

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title = "Identification and characterization of the high-affinity choline transporter",

abstract = "In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.",

author = "Takashi Okuda and Tatsuya Haga and Yoshikatsu Kanai and Hitoshi Endou and Takeshi Ishihara and Isao Katsura",

note = "Funding Information: We thank Y. Iino for the C. elegans N2 strain, Y. Kobayashi for help with basal forebrain preparations, A. Fire for pPD104.53, S. Yamashita for yeast choline-transporter cDNA, T. Suzuki and Y. Kirino for Torpedo electric lobe and its cDNA library, Y. Koyama for laterodorsal tegmental nucleus, K. Kameyama for suggestions and D. Saffen for English corrections. This work was supported by grants from Japan Science and Technology Corporation (CREST) and the Ministry of Japan Society for the Promotion of Science (Research for Future Program).",

year = "2000",

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doi = "10.1038/72059",

language = "English",

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pages = "120--125",

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T1 - Identification and characterization of the high-affinity choline transporter

AU - Okuda, Takashi

AU - Haga, Tatsuya

AU - Kanai, Yoshikatsu

AU - Endou, Hitoshi

AU - Ishihara, Takeshi

AU - Katsura, Isao

N1 - Funding Information: We thank Y. Iino for the C. elegans N2 strain, Y. Kobayashi for help with basal forebrain preparations, A. Fire for pPD104.53, S. Yamashita for yeast choline-transporter cDNA, T. Suzuki and Y. Kirino for Torpedo electric lobe and its cDNA library, Y. Koyama for laterodorsal tegmental nucleus, K. Kameyama for suggestions and D. Saffen for English corrections. This work was supported by grants from Japan Science and Technology Corporation (CREST) and the Ministry of Japan Society for the Promotion of Science (Research for Future Program).

PY - 2000/2

Y1 - 2000/2

N2 - In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

AB - In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho- 1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

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Identification and characterization of the high-affinity choline transporter

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