TY - JOUR
T1 - Identification of a novel zinc finger protein gene (ZNF298) in the GAP2 of human chromosome 21q
AU - Shibuya, Kazunori
AU - Kudoh, Jun
AU - Okui, Michiyo
AU - Shimizu, Nobuyoshi
N1 - Funding Information:
We thank Ms. Mio Mejima, Mie Furuhashi, and Junko Onoda for excellent technical assistance. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas “Medical Genome Science” from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT); and Fund for “Research for the Future” Program from the Japan Society for the Promotion of Science (JSPS) and MEXT.
PY - 2005/7/1
Y1 - 2005/7/1
N2 - We have isolated a novel zinc finger protein gene, designated ZNF298, as a candidate gene for a particular phenotype of Down syndrome or bipolar affective disorder (BPAD) which maps to human chromosome 21q22.3. ZNF298 gene consists of 25 exons spanning approximately 80 kb in a direction from the telomere to centromere. There are four kinds of transcripts that harbor three types of 3′ UTR. These four transcripts (ZNF298a, ZNF298b, ZNF298c, and ZNF298d) contain putative open reading frames encoding 1178, 1198, 555, and 515 amino acids, respectively. ZNF298 gene was ubiquitously expressed in various tissues at very low level. The protein motif analysis revealed that ZNF298 proteins contain a SET [Su(var)3-9, Enhancer-of-zeste, Trithorax] domain, multiple C2H2-type zinc finger (ZnF_C2H2) domains, several nuclear localization signals (NLSs), and PEST sequences. Nuclear localization of ZNF298 protein was confirmed by transfection of expression vector of GFP-tagged protein into two human cell lines. Interestingly, this gene crosses over a clone gap (GAP2) remaining in the band 21q22.3. We obtained the DNA fragments corresponding to GAP2 using ZNF298 cDNA sequence as anchor primers for PCR and determined its genomic DNA sequence.
AB - We have isolated a novel zinc finger protein gene, designated ZNF298, as a candidate gene for a particular phenotype of Down syndrome or bipolar affective disorder (BPAD) which maps to human chromosome 21q22.3. ZNF298 gene consists of 25 exons spanning approximately 80 kb in a direction from the telomere to centromere. There are four kinds of transcripts that harbor three types of 3′ UTR. These four transcripts (ZNF298a, ZNF298b, ZNF298c, and ZNF298d) contain putative open reading frames encoding 1178, 1198, 555, and 515 amino acids, respectively. ZNF298 gene was ubiquitously expressed in various tissues at very low level. The protein motif analysis revealed that ZNF298 proteins contain a SET [Su(var)3-9, Enhancer-of-zeste, Trithorax] domain, multiple C2H2-type zinc finger (ZnF_C2H2) domains, several nuclear localization signals (NLSs), and PEST sequences. Nuclear localization of ZNF298 protein was confirmed by transfection of expression vector of GFP-tagged protein into two human cell lines. Interestingly, this gene crosses over a clone gap (GAP2) remaining in the band 21q22.3. We obtained the DNA fragments corresponding to GAP2 using ZNF298 cDNA sequence as anchor primers for PCR and determined its genomic DNA sequence.
KW - Bipolar affective disorder
KW - Chromosome 21
KW - Down syndrome
KW - Genome sequencing
KW - SET domain
KW - Zinc finger protein
KW - cDNA
UR - http://www.scopus.com/inward/record.url?scp=19444372141&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19444372141&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.04.159
DO - 10.1016/j.bbrc.2005.04.159
M3 - Article
C2 - 15904895
AN - SCOPUS:19444372141
SN - 0006-291X
VL - 332
SP - 557
EP - 568
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -