Identification of genes associated with the astrocyte-specific gene Gfap during astrocyte differentiation

Kenji Ito; Tsukasa Sanosaka; Katsuhide Igarashi; Maky Ideta-Otsuka; Akira Aizawa; Yuichi Uosaki; Azumi Noguchi; Hirokazu Arakawa; Kinichi Nakashima; Takumi Takizawa

doi:10.1038/srep23903

Identification of genes associated with the astrocyte-specific gene Gfap during astrocyte differentiation

Kenji Ito, Tsukasa Sanosaka, Katsuhide Igarashi, Maky Ideta-Otsuka, Akira Aizawa, Yuichi Uosaki, Azumi Noguchi, Hirokazu Arakawa, Kinichi Nakashima, Takumi Takizawa

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20 Citations (Scopus)

Abstract

Chromosomes and genes are non-randomly arranged within the mammalian cell nucleus, and gene clustering is of great significance in transcriptional regulation. However, the relevance of gene clustering and their expression during the differentiation of neural precursor cells (NPCs) into astrocytes remains unclear. We performed a genome-wide enhanced circular chromosomal conformation capture (e4C) to screen for genes associated with the astrocyte-specific gene glial fibrillary acidic protein (Gfap) during astrocyte differentiation. We identified 18 genes that were specifically associated with Gfap and expressed in NPC-derived astrocytes. Our results provide additional evidence for the functional significance of gene clustering in transcriptional regulation during NPC differentiation.

Original language	English
Article number	23903
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep23903
Publication status	Published - 2016 Apr 4
Externally published	Yes

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title = "Identification of genes associated with the astrocyte-specific gene Gfap during astrocyte differentiation",

abstract = "Chromosomes and genes are non-randomly arranged within the mammalian cell nucleus, and gene clustering is of great significance in transcriptional regulation. However, the relevance of gene clustering and their expression during the differentiation of neural precursor cells (NPCs) into astrocytes remains unclear. We performed a genome-wide enhanced circular chromosomal conformation capture (e4C) to screen for genes associated with the astrocyte-specific gene glial fibrillary acidic protein (Gfap) during astrocyte differentiation. We identified 18 genes that were specifically associated with Gfap and expressed in NPC-derived astrocytes. Our results provide additional evidence for the functional significance of gene clustering in transcriptional regulation during NPC differentiation.",

author = "Kenji Ito and Tsukasa Sanosaka and Katsuhide Igarashi and Maky Ideta-Otsuka and Akira Aizawa and Yuichi Uosaki and Azumi Noguchi and Hirokazu Arakawa and Kinichi Nakashima and Takumi Takizawa",

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T1 - Identification of genes associated with the astrocyte-specific gene Gfap during astrocyte differentiation

AU - Ito, Kenji

AU - Sanosaka, Tsukasa

AU - Igarashi, Katsuhide

AU - Ideta-Otsuka, Maky

AU - Aizawa, Akira

AU - Uosaki, Yuichi

AU - Noguchi, Azumi

AU - Arakawa, Hirokazu

AU - Nakashima, Kinichi

AU - Takizawa, Takumi

PY - 2016/4/4

Y1 - 2016/4/4

N2 - Chromosomes and genes are non-randomly arranged within the mammalian cell nucleus, and gene clustering is of great significance in transcriptional regulation. However, the relevance of gene clustering and their expression during the differentiation of neural precursor cells (NPCs) into astrocytes remains unclear. We performed a genome-wide enhanced circular chromosomal conformation capture (e4C) to screen for genes associated with the astrocyte-specific gene glial fibrillary acidic protein (Gfap) during astrocyte differentiation. We identified 18 genes that were specifically associated with Gfap and expressed in NPC-derived astrocytes. Our results provide additional evidence for the functional significance of gene clustering in transcriptional regulation during NPC differentiation.

AB - Chromosomes and genes are non-randomly arranged within the mammalian cell nucleus, and gene clustering is of great significance in transcriptional regulation. However, the relevance of gene clustering and their expression during the differentiation of neural precursor cells (NPCs) into astrocytes remains unclear. We performed a genome-wide enhanced circular chromosomal conformation capture (e4C) to screen for genes associated with the astrocyte-specific gene glial fibrillary acidic protein (Gfap) during astrocyte differentiation. We identified 18 genes that were specifically associated with Gfap and expressed in NPC-derived astrocytes. Our results provide additional evidence for the functional significance of gene clustering in transcriptional regulation during NPC differentiation.

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Identification of genes associated with the astrocyte-specific gene Gfap during astrocyte differentiation

Abstract

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