Identification of salivary metabolites for oral squamous cell carcinoma and oral epithelial dysplasia screening from persistent suspicious oral mucosal lesions

Shigeo Ishikawa, David T.W. Wong, Masahiro Sugimoto, Frederico Omar Gleber-Netto, Feng Li, Michael Tu, Yong Zhang, David Akin, Mitsuyoshi Iino

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Objective: To identify salivary metabolite biomarkers to differentiate patients with oral squamous cell carcinoma and oral epithelial dysplasia (OSCC/OED) from those with persistent suspicious oral mucosal lesions (PSOML). Subjects and methods: Whole unstimulated saliva samples were collected from age-, sex-, and race-matched patients who had a lesion in the oral cavity and for whom open biopsies were performed. The patients included OSCC (n = 6), OED (n = 10), and PSOML (n = 32). Hydrophilic metabolites in saliva samples were comprehensively analyzed using capillary electrophoresis mass spectrometry. To evaluate the discrimination ability of a combination of multiple markers, a multiple logistic regression (MLR) model was developed to differentiate OSCC/OED from PSOML. Results: Six metabolites were significantly different in OSCC/OED compared with PSOML. From these six metabolites, ornithine, o-hydroxybenzoate, and ribose 5-phosphate (R5P) were used to develop the MLR model, which resulted in a high value for the area under receiver operating characteristic curve (AUC 0.871, 95% confidential interval (CI) 0.760–0.982; p < 0.001) to discriminate OSCC/OED from PSOML. Conclusions: This is the first study to identify salivary metabolites that discriminate OSCC/OED from PSOML rather than from healthy controls. The profiles of salivary metabolites were significantly different between OSCC/OED and PSOML. The ability to discriminate OSCC/OED from PSOML is important for dentists who are not oral surgery specialists. These salivary metabolites showed potential for non-invasive screening to discriminate OSCC/OED from PSOML. Clinical relevance: Salivary metabolites in this study showed potential for non-invasive screening to discriminate OSCC/OED from PSOML.

Original languageEnglish
Pages (from-to)3557-3563
Number of pages7
JournalClinical Oral Investigations
Volume23
Issue number9
DOIs
Publication statusPublished - 2019 Sept 1
Externally publishedYes

Keywords

  • Metabolites
  • Oral epithelial dysplasia
  • Oral squamous cell carcinoma
  • Saliva
  • Screening

ASJC Scopus subject areas

  • General Dentistry

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