Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries

Shinsuke Shibata; Yukiko Murota; Yoshinori Nishimoto; Mana Yoshimura; Toshihiro Nagai; Hideyuki Okano; Mikiko C. Siomi

doi:10.1007/978-1-4939-2851-4_12

Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specifi c proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specifi c target labeled with both fl uorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

Original language	English
Pages (from-to)	163-178
Number of pages	16
Journal	Methods in Molecular Biology
Volume	1328
DOIs	https://doi.org/10.1007/978-1-4939-2851-4_12
Publication status	Published - 2015

Keywords

EM-ISH
Electron microscopic in situ hybridization
Electron microscopy
Flam
Fluorescence immunohistochemistry
ISH
Immuno-electron microscopy
In situ hybridization
PIWI-interacting RNAs
PiRNAs
Yb
Zuc

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2851-4_12

Cite this

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title = "Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries",

abstract = "Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specifi c proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specifi c target labeled with both fl uorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.",

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doi = "10.1007/978-1-4939-2851-4_12",

language = "English",

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AU - Shibata, Shinsuke

AU - Murota, Yukiko

AU - Nishimoto, Yoshinori

AU - Yoshimura, Mana

AU - Nagai, Toshihiro

AU - Okano, Hideyuki

AU - Siomi, Mikiko C.

PY - 2015

Y1 - 2015

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KW - EM-ISH

KW - Electron microscopic in situ hybridization

KW - Electron microscopy

KW - Flam

KW - Fluorescence immunohistochemistry

KW - ISH

KW - Immuno-electron microscopy

KW - In situ hybridization

KW - PIWI-interacting RNAs

KW - PiRNAs

KW - Yb

KW - Zuc

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JO - Methods in Molecular Biology

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Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries

Abstract

Keywords

ASJC Scopus subject areas

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