Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries

Shinsuke Shibata; Yukiko Murota; Yoshinori Nishimoto; Mana Yoshimura; Toshihiro Nagai; Hideyuki Okano; Mikiko C. Siomi

doi:10.1007/978-1-4939-2851-4_12

Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

Original language	English
Title of host publication	Drosophila Oogenesis
Subtitle of host publication	Methods and Protocols
Publisher	Springer New York
Pages	163-178
Number of pages	16
ISBN (Electronic)	9781493928514
ISBN (Print)	9781493928507
DOIs	https://doi.org/10.1007/978-1-4939-2851-4_12
Publication status	Published - 2015 Aug 31

Keywords

EM-ISH
Electron microscopic in situ hybridization
Electron microscopy
Flam
Fluorescence immunohistochemistry
Immuno-electron microscopy
In situ hybridization
Ish
PIWI-interacting RNAs
Yb
Zuc
piRNAs

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
Medicine(all)

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10.1007/978-1-4939-2851-4_12

Cite this

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title = "Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries",

abstract = "Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.",

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AU - Shibata, Shinsuke

AU - Murota, Yukiko

AU - Nishimoto, Yoshinori

AU - Yoshimura, Mana

AU - Nagai, Toshihiro

AU - Okano, Hideyuki

AU - Siomi, Mikiko C.

PY - 2015/8/31

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KW - Electron microscopic in situ hybridization

KW - Electron microscopy

KW - Flam

KW - Fluorescence immunohistochemistry

KW - Immuno-electron microscopy

KW - In situ hybridization

KW - Ish

KW - PIWI-interacting RNAs

KW - Yb

KW - Zuc

KW - piRNAs

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BT - Drosophila Oogenesis

PB - Springer New York

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Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries

Abstract

Keywords

ASJC Scopus subject areas

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