Immunohistochemical study of. GAMMA.-glutamyl transpeptidase with monoclonal antibodies. I Preparation and characteristics of monoclonal antibodies to. GAMMA.-glutamyl transpeptidase

Kenjiro Yasuda; Yamashita Shuji; Sadakazu Aiso; Masahide Shiozawa; Toshiko Komatsu

doi:10.1267/ahc.19.589

Immunohistochemical study of. GAMMA.-glutamyl transpeptidase with monoclonal antibodies. I Preparation and characteristics of monoclonal antibodies to. GAMMA.-glutamyl transpeptidase

Kenjiro Yasuda, Yamashita Shuji, Sadakazu Aiso, Masahide Shiozawa, Toshiko Komatsu

Department of Anatomy

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30 Citations (Scopus)

Abstract

Monoclonal antibodies of the IgG₁ subclass were prepared against rat kidney γ-glutamyl transpeptidase by the hybridoma technique. Eight hybrid clones produced antibodies against the enzyme. Finally two stable clones which produce specific antibodies were obtained. The antibodies specifically recognized a heavy subunit (Mr=51, 000) of the enzyme. Immunohistochemical examination revealed that the reaction was localized in the brush border as well as along the basolateral membrane of the proximal convolution of rat kidney and also in the epididymis and the pancreas of rats. Human kidney showed positive reaction, whereas no reaction was recognized in the kidneys of other species, such as mice, guinea pigs and rabbits.

Original language	English
Pages (from-to)	589-600
Number of pages	12
Journal	Acta Histochemica et Cytochemica
Volume	19
Issue number	5
DOIs	https://doi.org/10.1267/ahc.19.589
Publication status	Published - 1986

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biochemistry
Physiology
Histology
Cell Biology

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Immunohistochemical study of. GAMMA.-glutamyl transpeptidase with monoclonal antibodies. I Preparation and characteristics of monoclonal antibodies to. GAMMA.-glutamyl transpeptidase. / Yasuda, Kenjiro; Shuji, Yamashita; Aiso, Sadakazu et al.
In: Acta Histochemica et Cytochemica, Vol. 19, No. 5, 1986, p. 589-600.

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abstract = "Monoclonal antibodies of the IgG1 subclass were prepared against rat kidney γ-glutamyl transpeptidase by the hybridoma technique. Eight hybrid clones produced antibodies against the enzyme. Finally two stable clones which produce specific antibodies were obtained. The antibodies specifically recognized a heavy subunit (Mr=51, 000) of the enzyme. Immunohistochemical examination revealed that the reaction was localized in the brush border as well as along the basolateral membrane of the proximal convolution of rat kidney and also in the epididymis and the pancreas of rats. Human kidney showed positive reaction, whereas no reaction was recognized in the kidneys of other species, such as mice, guinea pigs and rabbits.",

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AU - Shiozawa, Masahide

AU - Komatsu, Toshiko

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Immunohistochemical study of. GAMMA.-glutamyl transpeptidase with monoclonal antibodies. I Preparation and characteristics of monoclonal antibodies to. GAMMA.-glutamyl transpeptidase

Abstract

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