Imprinting of human GRB10 and its mutations in two patients with Russell-Silver syndrome

Hiroshi Yoshihashi, Katsuhiro Maeyama, Rika Kosaki, Tsutomu Ogata, Masato Tsukahara, Yu Ichi Goto, Jun Ichi Hata, Nobutake Matsuo, Robert J. Smith, Kenjiro Kosaki

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81 Citations (Scopus)


Documentation of maternal uniparental disomy of chromosome 7 in 10% of patients with Russell-Silver syndrome (RSS), characterized by prenatal and postnatal growth retardation and dysmorphic features, has suggested the presence of an imprinted gene on chromosome 7 whose mutation is responsible for the RSS phenotype. Human GRB10 on chromosome 7, a homologue of the mouse imprinted gene Grb10, is a candidate, because GRB10 has a suppressive effect on growth, through its interaction with either the IGF-I receptor or the GH receptor, and two patients with RSS were shown to have a maternally derived duplication of 7p11-p13, encompassing GRB10. In the present study, we first demonstrated that the GRB10 gene is also monoallelically expressed in human fetal brain tissues and is transcribed from the maternally derived allele in somatic-cell hybrids. Hence, human GRB10 is imprinted. A mutation analysis ofGRB10 in 58 unrelated patients with RSS identified, within the N-terminal domain of the protein, a P95S substitution in two patients with RSS. In these two cases, the mutant allele was inherited from the mother. The fact that monoallelic GRB10 expression was observed from the maternal allele in this study suggests but does not prove that these maternally transmitted mutant alleles contribute to the RSS phenotype.

Original languageEnglish
Pages (from-to)476-482
Number of pages7
JournalAmerican Journal of Human Genetics
Issue number2
Publication statusPublished - 2000 Aug

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)


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