TY - JOUR
T1 - Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis
AU - Kato, S.
AU - Ishii, H.
AU - Kano, S.
AU - Hagihara, S.
AU - Todoroki, T.
AU - Nagata, S.
AU - Takahashi, H.
AU - Nagasaka, M.
AU - Sato, J.
AU - Tsuchiya, M.
PY - 1984
Y1 - 1984
N2 - We describe an improved method for determination of alcohol dehydrogenase (EC 1.1.1.1) activity in 60 μL of human serum, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine NaOH buffer (pH 9.0) at 37°C in a centrifugal analyzer. The final concentration of NAD+ was 10 mmol/L and ethanol was 20 mmol/L. The dilution curve was linear with enzyme activity up to 200 U/L, and results by this method correlated well with those by a manual method (N Engl J Med 279: 241-248, 1968). Within-run precision (CV) was 0.9 to 8.2% over the range of 4.5 to 88.1 U/L, and day-to-day precision was 5.4 to 5.6%. In sera from 198 healthy individuals, mean alcohol dehydrogenase activity was 1.6 (SD 1.2, range 0-5) U/L. To evaluate the clinical utility of determining alcohol dehydrogenase, we measured the activity of alanine aminotransferase and alcohol dehydrogenase in sera from 470 patients with various diseases in our hospital, and found that results for the two enzymes did not correlate well.
AB - We describe an improved method for determination of alcohol dehydrogenase (EC 1.1.1.1) activity in 60 μL of human serum, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine NaOH buffer (pH 9.0) at 37°C in a centrifugal analyzer. The final concentration of NAD+ was 10 mmol/L and ethanol was 20 mmol/L. The dilution curve was linear with enzyme activity up to 200 U/L, and results by this method correlated well with those by a manual method (N Engl J Med 279: 241-248, 1968). Within-run precision (CV) was 0.9 to 8.2% over the range of 4.5 to 88.1 U/L, and day-to-day precision was 5.4 to 5.6%. In sera from 198 healthy individuals, mean alcohol dehydrogenase activity was 1.6 (SD 1.2, range 0-5) U/L. To evaluate the clinical utility of determining alcohol dehydrogenase, we measured the activity of alanine aminotransferase and alcohol dehydrogenase in sera from 470 patients with various diseases in our hospital, and found that results for the two enzymes did not correlate well.
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U2 - 10.1093/clinchem/30.11.1817
DO - 10.1093/clinchem/30.11.1817
M3 - Article
C2 - 6386226
AN - SCOPUS:0021732087
SN - 0009-9147
VL - 30
SP - 1817
EP - 1820
JO - Clinical Chemistry
JF - Clinical Chemistry
IS - 11
ER -
