Abstract
Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.
| Original language | English |
|---|---|
| Pages (from-to) | 354-361 |
| Number of pages | 8 |
| Journal | Materials Science and Engineering C |
| Volume | 78 |
| DOIs | |
| Publication status | Published - 2017 Sept 1 |
Keywords
- Embryonic stem cell
- Feeder-free culture
- Pluripotent stem cell
- Surface modification
- UV/ozone treatment
- X-ray photoelectron spectroscopy
ASJC Scopus subject areas
- General Materials Science
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering
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